Phospholipids and their derivatives as mitogen and motogen of budding tunicates.

Phospholipids and their derivatives as mitogen and motogen of budding tunicates. Research Abstract Details 

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Phospholipids and their derivatives as mitogen and motogen of budding tunicates. Abstract Text:

Kazuya Arai,Satoshi Yoshida,Masanao Nakatani,Shigeki Fujiwara,Toshitsugu Yubisui,Kazuo Kawamura,

In order to discover novel invertebrate cytokines from the budding tunicate, Polyandrocarpa misakiensis, we treated the water-insoluble fraction of tunicate homogenates with trypsin. The extracts showed remarkable activities to promote the growth and motility of tunicate cells. The activities were heat-stable and proteinase K-resistant. After anion exchange chromatography, the activities were eluted with detergents such as 0.1% deoxycholic acid. The Fourier transform infrared spectrum indicated large amounts of fatty acids and phospholipids instead of polypeptides in the extracts. Consistently, the activities were extractable with organic solvents such as chloroform. Long chains of n-3 polyunsaturated free fatty acids (FFA), phosphatidylinositol (PI), phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylserine (PS) were the major components in the lipid-soluble fraction. A cDNA for FFA-releasing enzyme phospholipase A(2) (PLA(2)) was cloned. The expression of this gene could be seen in epidermal cells during budding. The recombinant protein, as in the case of the authentic PLA2, preferred PC and PE as substrates, followed by PS and PI. The resultant FFAs only promoted cell growth, while the remaining lysophospholipids stimulated cell motility. The former contained unsaturated fatty acids (C18:1, C20:5, and C22:6) while the latter did not, suggesting that unsaturated fatty acids are responsible for mitogenic activity in tunicate cells. These results show for the first time that phospholipids and their derivatives are bio-mediators promoting cell growth and cell motility in invertebrates.

Phospholipids and their derivatives as mitogen and motogen of budding tunicates. Publishing Authors By Initials

K Arai,S Yoshida,M Nakatani,S Fujiwara,T Yubisui,K Kawamura,

For similar animals: chordata: chordata, nonvertebrate: urochordata research abstracts see: animals: chordata: chordata, nonvertebrate: urochordata research

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Phospholipids and their derivatives as mitogen and motogen of budding tunicates. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 135

Page Numbers: 71-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2004

Phospholipids and their derivatives as mitogen and motogen of budding tunicates. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Phospholipids and their derivatives as mitogen and motogen of budding tunicates. Keywords Mesh Terms:

KEYWORDS: Urochordata

MESH TERMS: growth & development

Chemical & Substance for Abstract: Phospholipids and their derivatives as mitogen and motogen of budding tunicates. Information

Substance Name: Phospholipids

Registry Number: 0

Grant and Affiliation Information for Phospholipids and their derivatives as mitogen and motogen of budding tunicates.

AFFILIATION: Laboratory of Cellular and Molecular Biotechnology, Faculty of Science, Kochi University, Kochi 780-8520.

Country: Japan

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MEDLINETA: J Biochem

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