Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death.

Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death. Abstract Text:

Hong-Gang Yu,Yao-Wei Ai,Liang-Liang Yu,Xiao-Dong Zhou,Jin Liu,Jun-Hua Li,Xi-Ming Xu,Song Liu,Jing Chen,Fen Liu,Yuan-Ling Qi,Quanjun Deng,Jun Cao,Shi-Quan Liu,He-Sheng Luo,Jie-Ping Yu,Hong-Gang Yu,Yao-Wei Ai,Liang-Liang Yu,Xiao-Dong Zhou,Jin Liu,Jun-Hua Li,Xi-Ming Xu,Song Liu,Jing Chen,Fen Liu,Yuan-Ling Qi,Quanjun Deng,Jun Cao,Shi-Quan Liu,He-Sheng Luo,Jie-Ping Yu,

The major obstacle to successful treatment of gastric cancer is chemotherapy resistance. Our study was designed to investigate the role of phosphoinositide 3-kinase (PI3K)/Akt pathway in the development of chemoresistance in gastric cancer. In the present study, elevated Akt expression and Akt phosphorylation (Ser 473), as well as decreased PTEN expression were observed in 28 cases of gastric cancer tissues. Etoposide and doxorubicin stimulated Akt and PI3K activities in 2 gastric cancer cell lines (BGC-823 and SGC-7901), and the activities were concentration and time-dependent. Up-regulation of PTEN expression in BGC-823 cells by PEAK8-PTEN transient transfection obviously decreased the basal and anticancer drugs induced Akt activities, then sensitized BGC-823 cells to etoposide and doxorubicin. Pretreatment of BGC-823 and SGC-7901 cells with wortmannin, a PI3K inhibitor, attenuated cells's resistance to etoposide and doxorubicin. In addition, pretreatment of wortmannin blocked etoposide and doxorubicin induced IkappaB-alpha degradation, NFkappaB activation, phosphorylation of Akt, MDM-2 and forkhead transcription factors. Wortmannin pretreatment also promoted the accumulation of p27/Kip, but inhibited the Mcl-1 expression. Furthermore, wortmannin promoted etoposide and doxorubicin induced caspase-3, caspase-9 activation and poly ADP-ribose polymerase cleavage. Taken together, the observations indicate the PI3K/Akt pathway plays an important role in the chemoresistance of gastric cancer cells. A new strategy for combined chemotherapy of gastric cancer should be designed to more specifically block PI3K/Akt pathway and then decrease the amount of resistant cells.

Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death. Publishing Authors By Initials

HG Yu,YW Ai,LL Yu,XD Zhou,J Liu,JH Li,XM Xu,S Liu,J Chen,F Liu,YL Qi,Q Deng,J Cao,SQ Liu,HS Luo,JP Yu,HG Yu,YW Ai,LL Yu,XD Zhou,J Liu,JH Li,XM Xu,S Liu,J Chen,F Liu,YL Qi,Q Deng,J Cao,SQ Liu,HS Luo,JP Yu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: International journal of cancer. Journal internati

VOLUME: 122

Page Numbers: 433-43

Journal Abbreviation: Int. J. Cancer

ISSN: 1097-0215

DAY: 15

MONTH: Jan

YEAR: 2008

Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 42124

Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death.

AFFILIATION: Department of Gastroenterology, Renmin Hospital of Wuhan Univeristy, Wuhan, China. yuhonggang@yahoo.com

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Int J Cancer

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Phosphoinositide 3-kinase/Akt pathway plays an important role in chemoresistance of gastric cancer cells against etoposide and doxorubicin induced cell death Related Publications

• An overview of vagal airway receptors.
• Element mixing distribution and structure feature of fusion zone in laser welding between different alloys and pure titanium.
• Endothelial function and carotid artery wall thickening in patients with early essential hypertension.
• Genetic factors underlying prostate cancer.
• Genotype-phenotype analysis for the polymorphic CA repeat in the insulin-like growth factor-I (IGF-I) gene.
• Na(+)/K(+) -ATPase as a marker for detecting pulmonary sensory receptors.
• RRR-alpha-tocopheryl succinate inhibits human gastric cancer SGC-7901 cell growth by inducing apoptosis and DNA synthesis arrest.
• The effects of vitamin E succinate on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.
• [A case of sepsis and multiple organ dysfunction syndrome complicated by Staphylococcus infection]
• [Anisodamine in prevention and treatment of sepsis of severely burned patients]
• [Association study on HLA-DP and -DQ allelic polymorphisms and nasopharyngeal carcinoma in the Han nationality in Hunan province]
• [Change of catecholamine in serum and hippocampus of rats after electromagnetic irradiation and the selection of protective site]
• [Changes of phosphorylation states of Cx43 protein in Chinese hamster lung cells induced by SiO2]
• [Double uterus and hydatidiform mole in the malformed uterus: report of one case]
• [Early diagnosis of pancreatic cancer is essential to improving the effect of treatment]
• [Effect of oxidated low-density lipoprotein on early apoptosis of vascular smooth muscle cell]
• [Extinguishment of harmful algae by organo-clay]
• [Humoral immune response in mice to hybrid nucleic acid vaccines containing Plasmodium falciparum merozoite surface protein 1 block 17-based gene]
• [PCR in detecting the correlation between infection of HBV and cholelithiasis]
• [Post-mastectomy radiotherapy with different fractionated dose schemes in early breast cancer]
• [Primary extranodal lymphomas: analyses of clinical characteristics and misdiagnoses of 139 cases]
• [Relationship between Helicobacter pylori infection and expression of c-myc, Bcl-2, and Bax protein in different gastric mucosa lesions]
• [Researches in forensic biomechanics]
• [Study on antitumor effect and mechanism of aloe polysaccharides]
• [Study on the genic polymorphism of HLA-DRB1 in a population in the north of China]
• [The effect of radix Pseudostellariae from 8 habitats on spleen-deficiency and immunologic function]
• [The expression and activity of caspase-8 in the process of vitamin E succinate-induced apoptosis in human gastric carcinoma SGC-7901 cells]
• [Treatment of burns combined injury]
• [Wavelet feature extraction and classification of Doppler ultrasound blood flow signals]
• [p15 gene expression in acute lymphoblastic leukemia cell line Molt4 induced by arsenic trioxide]