Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging.

Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging. Research Abstract Details 

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Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging. Abstract Text:

Audra G Sostarecz,Carolyn M McQuaw,Andrew G Ewing,Nicholas Winograd,

Coexisting liquid phases of model membrane systems are chemically identified using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS). The systems studied were Langmuir-Blodgett (LB) model membranes of cholesterol (CH) with two different phospholipids, one a major component in the outer plasma membrane bilayer leaflet (dipalmitoylphosphatidylcholine (PC)) and the other a major component in the inner leaflet (dipalmitoylphosphatidylethanolamine (PE)). Binary mixtures of CH with each of the phospholipids were investigated, as well as a ternary system. A single homogeneous phase is evident for PC/CH, whereas both systems containing PE show lateral heterogeneity with phospholipid-rich and CH-rich regions. The interaction between CH and the two phospholipids differs due to the disparity between the phospholipid headgroups. Imaging TOF-SIMS offers a novel opportunity to chemically identify and differentiate the specific membrane locations of CH and phospholipid in membrane regions without the use of fluorescent dyes. This unique imaging method has been used to demonstrate the formation of micrometer-size CH domains in phosphatidylethanolamine-rich systems and is further evidence suggesting that CH may facilitate transport and signaling across the two leaflets of the plasma membrane.

Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging. Publishing Authors By Initials

AG Sostarecz,CM McQuaw,AG Ewing,N Winograd,

For similar phosphatidic acids: glycerophospholipids: phosphatidylethanolamines research abstracts see: phosphatidic acids: glycerophospholipids: phosphatidylethanolamines research

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Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 13882-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 3

MONTH: Nov

YEAR: 2004

Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging. Keywords Mesh Terms:

KEYWORDS: Phosphatidylethanolamines

MESH TERMS: metabolism

Chemical & Substance for Abstract: Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging. Information

Substance Name: Cholesterol

Registry Number: 57-88-5

Grant and Affiliation Information for Phosphatidylethanolamine-induced cholesterol domains chemically identified with mass spectrometric imaging.

AFFILIATION: Department of Chemistry, The Pennsylvania State University, 104 Chemistry Building, University Park, Pennsylvania, 16802, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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