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Phosphatidylcholine transfer activity in human erythrocyte hemolysate.

Phosphatidylcholine transfer activity in human erythrocyte hemolysate. Research Abstract Details 

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  • Phosphatidylcholine transfer activity in human erythrocyte hemolysate. Abstract Text:

    k kurodaK Kuroda,s ohnishiS Ohnishi,

    Phosphatidylcholine (PC) transfer activity was found in human erythrocyte hemolysate. The transfer activity was assayed by the ESR peak height increase when spin-labeled PC vesicles were incubated with egg yolk PC vesicles. The transfer activity was isolated from hemoglobin by an ion exchange chromatography followed by gel filtration. The partial purification resulted in a 405-fold increase in the specific transfer activity compared with that of the hemolysate. The molecular weight of the PC transfer protein was estimated to be 23,000 by gel filtration. The transfer activity was inactivated by heat-treatment at 75 degrees C for 10 min. Phosphatidylserine vesicles strongly inhibited the activity. Half-maximal inhibition occurred on addition of 0.24 mol% of phosphatidylserine vesicles to the incubation mixture. Ca2+ restored the activity. The transfer protein was quite similar to the PC transfer protein obtained from bovine liver cytosol.

    Phosphatidylcholine transfer activity in human erythrocyte hemolysate. Publishing Authors By Initials

    k kurodaK Kuroda,s ohnishiS Ohnishi,

    For similar proteins: carrier proteins: phospholipid transfer proteins research abstracts see: proteins: carrier proteins: phospholipid transfer proteins research

    PUBMED ID PMID:

    MEDLINE DATE:

    Phosphatidylcholine transfer activity in human erythrocyte hemolysate. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 94

    Page Numbers: 1809-13

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Dec

    YEAR: 1983

    Phosphatidylcholine transfer activity in human erythrocyte hemolysate. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Phosphatidylcholine transfer activity in human erythrocyte hemolysate. Keywords Mesh Terms:

    KEYWORDS: Phospholipid Transfer Proteins

    MESH TERMS: blood

    Chemical & Substance for Abstract: Phosphatidylcholine transfer activity in human erythrocyte hemolysate. Information

    Substance Name: Phospholipid Transfer Proteins

    Registry Number: 0

    Grant and Affiliation Information for Phosphatidylcholine transfer activity in human erythrocyte hemolysate.

    AFFILIATION:

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    GRANT:

    ACRONYM:

    MEDLINETA: J Biochem

    REFSOURCE:

    DATABASENAME:

    ACCESSION NUMBER:

    Number Hits: 0

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