Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells.

Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells. Abstract Text:

S Y Han,M S Lee,H R Kim,S H Baek,D H Ahn,H S Chae,R H Erickson,M H Sleisenger,Y S Kim,

Phorbol esters such as phorbol 12-myristate 13-acetate (PMA) have been reported to modulate diverse cellular responses through signal transduction pathways including the protein kinase C (PKC) pathway. In the present study, we sought to determine the effect of PMA on mucin gene expression and on the biological properties of a human colon cancer cell line, HM3. The cells were treated for 8 and 24 h with various concentrations of PMA and total RNA was extracted and Northern and slot blot analyses were carried out using MUC2, MUC3 and MUC5AC mucin cDNA probes to assess the steady state levels of mRNA. Spent media were collected and the level of cancer associated carbohydrate antigens (T, Tn, sialyl Tn, sialyl Lex, and sialyl Lea) and matrix-degrading metalloproteinase (MMPs) activity were examined. Trypsinized cells were used for assessing in vitro invasion, motility and adhesion to matrigel. Our results showed that PMA caused upregulation of steady state mRNA levels of MUC2, MUC3 and MUC5AC which was inhibited after treatment with protein synthesis inhibitors. Calphostin C, a highly specific inhibitor of protein kinase C significantly inhibited the PMA induced induction of mRNA levels of MUC2, MUC3, and MUC5AC. The levels of all cancer-associated mucin carbohydrate antigens examined in the media were increased by PMA treatment. PMA also caused an increase in MMPs activity and in in vitro invasion and motility properties, but did not affect adhesion of HM3 cells to matrigel. Thus, PMA caused a significant increase in the expression of all three mucin genes through signaling pathways involving protein kinase C and increased secretion of mucin associated carbohydrate antigens. These changes were associated with increases in MMP activity as well as by increases in the invasive and motility properties of HM3 colon cancer cells. These data suggest that protein kinase C signaling pathways may be involved in mucin gene regulation and in modulating the invasive and metastatic properties of colon cancer cells.

Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells. Publishing Authors By Initials

SY Han,MS Lee,HR Kim,SH Baek,DH Ahn,HS Chae,RH Erickson,MH Sleisenger,YS Kim,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

PUBMED ID PMID:

MEDLINE DATE:

Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: International journal of oncology

VOLUME: 17

Page Numbers: 487-94

Journal Abbreviation: Int. J. Oncol.

ISSN: 1019-6439

DAY: 1

MONTH: Sep

YEAR: 2000

Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9306042

Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: metabolism

Chemical & Substance for Abstract: Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells. Information

Substance Name: Metalloendopeptidases

Registry Number: EC 3.4.24.-

Grant and Affiliation Information for Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells.

AFFILIATION: Gastrointestinal Research Laboratory, Department of Veterans Affairs Medical Center, San Francisco, CA 94121, USA.

Country: GREECE

AGENCY: United States NCI

GRANT: CA24321

ACRONYM: CA

MEDLINETA: Int J Oncol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells Related Publications

• Aberrant expression of MUC3 and MUC4 membrane-associated mucins and sialyl Le(x) antigen in pancreatic intraepithelial neoplasia.
• Aberrant expression of MUC5AC and MUC6 gastric mucins and sialyl Tn antigen in intraepithelial neoplasms of the pancreas.
• Advanced capabilities for future light sources.
• Aging of the population and the subsequent increase in the mistreatment of the elderly in Korea.
• Alkali-catalyzed beta-elimination of periodate-oxidized glycans: a novel method of chemical deglycosylation of mucin gene products in paraffin embedded sections.
• Altered mucin core peptide immunoreactivity in the colon polyp-carcinoma sequence.
• Basic fibroblast growth factor-induced translocation of p21-activated kinase to the membrane is independent of phospholipase C-gamma1 in the differentiation of PC12 cells.
• Biological properties and expression of mucins in 5-fluorouracil resistant HT29 human colon cancer cells.
• Cholangiocarcinomas arising in cirrhosis and combined hepatocellular-cholangiocellular carcinomas share apomucin profiles.
• Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans.
• Drynariae Rhizoma promotes osteoblast differentiation and mineralization in MC3T3-E1 cells through regulation of bone morphogenetic protein-2, alkaline phosphatase, type I collagen and collagenase-1.
• Elderly Women's Grief in Korea.
• Expression of MUC2, MUC5AC and MUC6 apomucins in carcinoma, dysplasia and non-dysplastic epithelia of the gallbladder.
• Goblet cell-specific expression mediated by the MUC2 mucin gene promoter in the intestine of transgenic mice.
• Identification and characterization of the MUC2 (human intestinal mucin) gene 5'-flanking region: promoter activity in cultured cells.
• Immunohistochemical study of mucin carbohydrates and core proteins in hepatolithiasis and cholangiocarcinoma.
• Induction of mucin gene expression in middle ear of rats by tumor necrosis factor-alpha: potential cause for mucoid otitis media.
• Initiation of transcription of the MUC3A human intestinal mucin from a TATA-less promoter and comparison with the MUC3B amino terminus.
• MUC3 human intestinal mucin. Analysis of gene structure, the carboxyl terminus, and a novel upstream repetitive region.
• Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells.
• Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor.
• Monoclonal antibodies against partially deglycosylated colon cancer mucin that recognize Tn antigen.
• Mouse intestinal goblet cells expressing SV40 T antigen directed by the MUC2 mucin gene promoter undergo apoptosis upon migration to the villi.
• Optical design of the U7 undulator beamline at the Pohang Light Source.
• Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells.
• Phorbol 12-myristate 13-acetate up-regulates the transcription of MUC2 intestinal mucin via Ras, ERK, and NF-kappa B.
• Stent-graft placement in a traumatic internal carotid-internal jugular fistula and pseudoaneurysm.
• Stereotactic aspiration of intracerebral haematoma: significance of surgical timing and haematoma volume reduction.
• Study of the aging perception.
• The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42.