Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay.

Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay. Research Abstract Details 

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Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay. Abstract Text:

Akihisa Okumura,Ikuya Tsuge,Tetsuo Kubota,Hirokazu Kurahashi,Jun Natsume,Tamiko Negoro,Kazuyoshi Watanabe,Akihisa Okumura,Ikuya Tsuge,Tetsuo Kubota,Hirokazu Kurahashi,Jun Natsume,Tamiko Negoro,Kazuyoshi Watanabe,Akihisa Okumura,Ikuya Tsuge,Tetsuo Kubota,Hirokazu Kurahashi,Jun Natsume,Tamiko Negoro,Kazuyoshi Watanabe,

We evaluated drug-specific T cell responses in a patient with refractory partial seizures and paroxysmal kinesigenic choreoathetosis successfully treated with clinical desensitization to phenytoin. Drug-induced lymphocyte transformation test before desensitization was negative with a stimulation index of 130%. The frequencies and cytokine-producing phenotypes of phenytoin-specific T cells were examined simultaneously by using a carboxyfluorescein succinimidyl ester (CFSE) dilution assay. Before desensitization, the proportion of CFSE(low) CD4+ cells in whole CD4+ was 3.09%; 13.6% of CFSE(low) CD4+ cells were stained with anti-interferon gamma antibody. After desensitization, phenytoin-specific CFSE(low) CD4+ cells decreased to background level. These results indicate that CFSE dilution assay will be useful for the diagnosis and monitoring of drug hypersensitivity.

Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay. Publishing Authors By Initials

A Okumura,I Tsuge,T Kubota,H Kurahashi,J Natsume,T Negoro,K Watanabe,A Okumura,I Tsuge,T Kubota,H Kurahashi,J Natsume,T Negoro,K Watanabe,A Okumura,I Tsuge,T Kubota,H Kurahashi,J Natsume,T Negoro,K Watanabe,

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Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: European journal of paediatric neurology : EJPN :

VOLUME: 11

Page Numbers: 385-8

Journal Abbreviation: Eur. J. Paediatr. Neurol.

ISSN: 1090-3798

DAY: 10

MONTH: 04

YEAR: 2007

Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay. Information

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LANGUAGE: eng

NlmUniqueID: 9715169

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Grant and Affiliation Information for Phenytoin desensitization monitored by antigen specific T cell response using carboxyfluorescein succinimidyl ester dilution assay.

AFFILIATION: Department of Pediatrics, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan; Department of Pediatrics, Nagoya University Graduate School of Medicine, Japan.

Country: England

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MEDLINETA: Eur J Paediatr Neurol

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