Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture.

Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture. Abstract Text:

Jennifer L Hoard,Donald B Hoover,Robert Wondergem,Jennifer L Hoard,Donald B Hoover,Robert Wondergem,

Intrinsic cardiac neurons are core elements of a complex neural network that serves as an important integrative center for regulation of cardiac function. Although mouse models are used frequently in cardiovascular research, very little is known about mouse intrinsic cardiac neurons. Accordingly, we have dissociated neurons from adult mouse heart, maintained these cells in culture, and defined their basic phenotypic properties. Neurons in culture were primarily unipolar, and 89% had prominent neurite outgrowth after 3 days (longest neurite length of 258 +/- 20 mum, n = 140). Many neurites formed close appositions with other neurons and nonneuronal cells. Neurite outgrowth was drastically reduced when neurons were kept in culture with a majority of nonneural cells eliminated. This finding suggests that nonneuronal cells release molecules that support neurite outgrowth. All neurons in coculture showed immunoreactivity for a full complement of cholinergic markers, but about 21% also stained for tyrosine hydroxylase, as observed previously in sections of intrinsic cardiac ganglia from mice and humans. Whole cell patch-clamp recordings demonstrated that these neurons have voltage-activated sodium current that is blocked by tetrodotoxin and that neurons exhibit phasic or accommodating patterns of action potential firing during a depolarizing current pulse. Several neurons exhibited a fast inward current mediated by nicotinic ACh receptors. Collectively, this work shows that neurons from adult mouse heart can be maintained in culture and exhibit appropriate phenotypic properties. Accordingly, these cultures provide a viable model for evaluating the physiology, pharmacology, and trophic factor sensitivity of adult mouse cardiac parasympathetic neurons.

Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture. Publishing Authors By Initials

JL Hoard,DB Hoover,R Wondergem,JL Hoard,DB Hoover,R Wondergem,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Cell physiology

VOLUME: 293

Page Numbers: C1875-83

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 3

MONTH: 10

YEAR: 2007

Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture.

AFFILIATION: Dept. of Physiology, P.O. Box 70,576, James H. Quillen College of Medicine, East Tennessee State Univ., Johnson City, TN 37614-1708. wonderge@etsu.edu).

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Physiol Cell Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture Related Publications

• Baló concentric sclerosis: MR diagnosis.
• Phenotypic properties of adult mouse intrinsic cardiac neurons maintained in culture.
• Thermal enhancement of the cell killing effect of X-irradiation in mammalian cells in vitro and in a transplantable mouse tumor: influence of pH, thermotolerance, hypoxia, or misonidazole.