Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations.

Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations. Research Abstract Details 

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Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations. Abstract Text:

Amy L Bishop,Faiza A Rab,Edward R Sumner,Simon V Avery,

Individual cells within isogenic microbial cultures exhibit phenotypic heterogeneity, an issue that is attracting intense interest. Heterogeneity could confer benefits, in generating variant subpopulations that may be better equipped to persist during perturbation. We tested this hypothesis by comparing the survival of wild-type Saccharomyces cerevisiae with that of mutants which are considered stress-sensitive but which, we demonstrate, also have increased heterogeneity. The mutants (e.g. vma3, ctr1, sod1) exhibited the anticipated sensitivities to intermediate doses of nickel, copper, alkaline pH, menadione or paraquat. However, enhanced heterogeneity meant that the resistances of individual mutant cells spanned a broad range, and at high stress occasional-cell survival in most of these populations overtook that of the wild type. Green fluorescent protein (GFP) reporter studies showed that this heterogeneity-dependent advantage was not related to perturbation of buffered gene expression. Deletion strain screens combined with other approaches revealed that vacuolar alkalinization resulting from loss of Vma-dependent vacuolar H(+)-ATPase activity was not the cause of vma mutants' net stress sensitivities. An alternative Vma-dependent resistance mechanism was found to suppress an influence of variable vacuolar pH on the metal resistances of individual wild-type cells. In addition to revealing new mechanisms of heterogeneity generation, the results demonstrate experimentally a benefit under adverse conditions that arises specifically from heterogeneity, and in populations conventionally considered to be disadvantaged.

Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations. Publishing Authors By Initials

AL Bishop,FA Rab,ER Sumner,SV Avery,

For similar organic chemicals: hydrocarbons: hydrocarbons, cyclic: hydrocarbons, aromatic: polycyclic hydrocarbons, aromatic: naphthalenes: naphthoquinones: vitamin k: vitamin k 3 research abstracts see: organic chemicals: hydrocarbons: hydrocarbons, cyclic: hydrocarbons, aromatic: polycyclic hydrocarbons, aromatic: naphthalenes: naphthoquinones: vitamin k: vitamin k 3 research

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Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular microbiology

VOLUME: 63

Page Numbers: 507-20

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 14

MONTH: 12

YEAR: 2006

Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8712028

Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations. Keywords Mesh Terms:

KEYWORDS: Vitamin K 3

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations. Information

Substance Name: Vacuolar Proton-Translocating ATPases

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Phenotypic heterogeneity can enhance rare-cell survival in 'stress-sensitive' yeast populations.

AFFILIATION: School of Biology, Institute of Genetics, University of Nottingham, University Park, Nottingham NG7 2RD, UK.

Country: England

AGENCY: United States NIGMS

GRANT: R01 GM57945

ACRONYM: GM

MEDLINETA: Mol Microbiol

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