Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana.

Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. Research Abstract Details 

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Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. Abstract Text:

Dayana Rodriguez-Contreras,Xiuhong Feng,Kristie M Keeney,H G Archie Bouwer,Scott M Landfear,

Glucose is a major source of energy and carbon in promastigotes of Leishmania mexicana, and its uptake is mediated by three glucose transporters whose genes are encoded within a single cluster. A null mutant in which the glucose transporter gene cluster was deleted by homologous gene replacement was generated previously and shown to grow more slowly than wild type promastigotes but not to be viable as amastigotes in primary tissue culture macrophages or in axenic culture. Further phenotypic characterization demonstrates that the null mutant is unable to import glucose, mannose, fructose, or galactose and that each of the three glucose transporter isoforms, LmGT1, LmGT2, and LmGT3, is capable of transporting each of these hexoses. Complementation of the null mutant with each isoform is able to restore growth in each of the four hexoses to wild type levels. Null mutant promastigotes are reduced in size to about 2/3 the volume of wild type parasites. In addition, the null mutants are significantly more sensitive to oxidative stress than their wild type counterparts. These results underscore the importance of glucose transporters in the parasite life cycle and suggest reasons for their non-viability in the disease-causing amastigote stage.

Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. Publishing Authors By Initials

D Rodriguez-Contreras,X Feng,KM Keeney,HG Bouwer,SM Landfear,

For similar proteins: protozoan proteins research abstracts see: proteins: protozoan proteins research

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Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Molecular and biochemical parasitology

VOLUME: 153

Page Numbers: 9-18

Journal Abbreviation:

ISSN: 0166-6851

DAY: 18

MONTH: 01

YEAR: 2007

Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. Information

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LANGUAGE: eng

NlmUniqueID: 8006324

Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. Keywords Mesh Terms:

KEYWORDS: Protozoan Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana. Information

Substance Name: glucose transporter, Leishmania

Registry Number: 0

Grant and Affiliation Information for Phenotypic characterization of a glucose transporter null mutant in Leishmania mexicana.

AFFILIATION: Department of Molecular Microbiology and Immunology, Oregon Health & Science University, Portland, OR 97239, USA.

Country: Netherlands

AGENCY: United States NIAID

GRANT: U01AI56446

ACRONYM: AI

MEDLINETA: Mol Biochem Parasitol

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