Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa.

Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa. Research Abstract Details 

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Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa. Abstract Text:

Ming-Wen Li,John D Biggers,Mehmet Toner,Stephen M Griffey,K C Kent Lloyd,Ming-Wen Li,John D Biggers,Mehmet Toner,Stephen M Griffey,K C Kent Lloyd,

Combination of evaporative drying and frozen storage at -80 degrees C has been used successfully to preserve hybrid B6D2F1 mouse spermatozoa. To determine whether this method can be applied equally well to inbred mice, spermatozoa of C57BL/6J and FVB/ NJ mice were evaporatively dried and stored for 1 mo at -80 degrees C before being used for intracytoplasmic sperm injection (ICSI) to produce live offspring. After weaning, 1 male and 1 female mouse from each litter were randomly selected at 8 wk of age for natural mating to produce live offspring. Results showed that spermatozoa from both inbred strains that had been evaporatively dried and subsequently stored at -80 degrees C could be used successfully to derive live, healthy, and reproductively sound offspring by ICSI. No significant differences were found in embryo transfer rate (number of pups born/number of embryos transferred), litter size, weaning rate, body weight, number of pathologic lesions, and amount of contamination by pathogens of mice produced by ICSI using evaporatively dried spermatozoa compared with mice produced by natural mating or by ICSI using fresh (that is, nonpreserved) spermatozoa. Progeny produced by mating mice generated from ICSI using evaporatively dried spermatozoa were normal. Therefore, spermatozoa from inbred mouse strains C57BL/6J and FVB/NJ can be preserved successfully after evaporative drying and frozen storage at -80 degrees C.

Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa. Publishing Authors By Initials

MW Li,JD Biggers,M Toner,SM Griffey,KC Lloyd,MW Li,JD Biggers,M Toner,SM Griffey,KC Lloyd,

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Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Comparative medicine

VOLUME: 57

Page Numbers: 469-75

Journal Abbreviation: Comp. Med.

ISSN: 1532-0820

DAY: 2

MONTH: Oct

YEAR: 2007

Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa. Information

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LANGUAGE: eng

NlmUniqueID: 100900466

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Grant and Affiliation Information for Phenotypic analysis of C57BL/6J and FVB/NJ mice generated using evaporatively dried spermatozoa.

AFFILIATION: Center for Comparative Medicine, School of Veterinary Medicine, University of California, Davis, CA, USA.

Country: United States

AGENCY: United States NCRR

GRANT: U42 RR14905

ACRONYM: RR

MEDLINETA: Comp Med

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