Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen.

Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen. Research Abstract Details 

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Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen. Abstract Text:

Nobutaka Hanagata,Taro Takemura,Akira Monkawa,Toshiyuki Ikoma,Junzo Tanaka,Nobutaka Hanagata,Taro Takemura,Akira Monkawa,Toshiyuki Ikoma,Junzo Tanaka,

Hydroxyapatite and type-I collagen are major components of bone matrix. We compared phenotype and gene expression pattern of osteoblast-like cells cultured on HAp and HAp with pre-adsorbed type-I collagen from neutral solutions (HAp/NCs) with those of tissue culture grade polystyrene (TCPS) and TCPS with the collagen (TCPS/NCs). In initial cell attachment, the cells tensely spread on TCPS, while loosely spread on TCPS/NCs, HAp, and HAp/NCs. The levels of expressed integrin alpha2 and alpha5 subunits were not significantly different among any surfaces. Although the cells on HAp/NCs directly attached with the same collagen molecules as TCPS/NCs, mineralization was observed in only TCPS/NCs. The basal substrates (TCPS and HAp) greatly influenced osteoblast maturation even in the surfaces with pre-adsorbed collagen, since mineralization was induced by TCPS, but not by HAp. Gene expression pattern analyzed with DNA microarray also supported greater influence of basal substrates than pre-adsorbed collagen. In addition, comprehensive gene expression analyses revealed that HAp and HAp/NCs specifically up-regulated Ly6a and Tmem37 genes, while down-regulated Ifitm5 gene.

Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen. Publishing Authors By Initials

N Hanagata,T Takemura,A Monkawa,T Ikoma,J Tanaka,N Hanagata,T Takemura,A Monkawa,T Ikoma,J Tanaka,

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Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part A

VOLUME: 83

Page Numbers: 362-71

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ISSN: 1549-3296

DAY: 24

MONTH: Nov

YEAR: 2007

Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen. Information

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LANGUAGE: eng

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AFFILIATION: Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan. HANAGATA.Nobutaka@nims.go.jp

Country: United States

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MEDLINETA: J Biomed Mater Res A

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