Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts.

Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts. Abstract Text:

Chun Wu,Hongxing Lei,Zhixiang Wang,Wei Zhang,Yong Duan,

Amyloid-associated diseases affect millions of people worldwide. Phenol red exhibits modest inhibition toward fibril formation of human Islet amyloid polypeptide (hIAPP) and its toxicity, which is associated with type II diabetes mellitus. However, the molecular level mechanisms of interactions remain elusive. The binding of phenol red molecules to the protofibrils of an amyloidogenic fragment (NFGAIL) of hIAPP has been investigated by molecular dynamics simulations with explicit solvent. The phenol red molecules were observed to bind primarily along either beta-sheet stacking or beta-strand directions. Through its three aromatic rings, the phenol red molecule preferentially interacted with the hydrophobic side chains of Phe, Leu, and Ile; and the polar sulfone and hydroxyl groups were mainly exposed in solvent. Thus, phenol red improves the solubility of the early protofibrils and represses further growth. Interestingly, there was no obvious preference toward the aromatic Phe residue in comparison to the hydrophobic Leu or Ile residues. The lack of binding along the hydrogen bond direction indicates that phenol red does not directly block the beta-sheet extension. Further free energy analysis suggested that a phenol red analog may potentially improve the binding affinity.

Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts. Publishing Authors By Initials

C Wu,H Lei,Z Wang,W Zhang,Y Duan,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research

PUBMED ID PMID:

MEDLINE DATE:

Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biophysical journal

VOLUME: 91

Page Numbers: 3664-72

Journal Abbreviation: Biophys. J.

ISSN: 0006-3495

DAY: 25

MONTH: 08

YEAR: 2006

Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370626

Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts. Keywords Mesh Terms:

KEYWORDS: Protein Conformation

MESH TERMS: chemistry

Chemical & Substance for Abstract: Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts. Information

Substance Name: Phenolsulfonphthalein

Registry Number: 143-74-8

Grant and Affiliation Information for Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts.

AFFILIATION: Genome Center and Department of Applied Science, University of California, Davis, California 95616, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM67168

ACRONYM: GM

MEDLINETA: Biophys J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts Related Publications

• An Automatic and Power Spectra-based Rotate Correcting Algorithm for Microarray Image.
• Challenges and opportunities in dystrophin-deficient cardiomyopathy gene therapy.
• Chiral selective effects of doxazosin enantiomers on blood pressure and urinary bladder pressure in anesthetized rats.
• Denatured-state ensemble and the early-stage folding of the G29A mutant of the B-domain of protein A.
• Diffuse Alveolar Lesion in BALB/c Mice Induced with Human Reovirus BYD1 Strain and its Potential Relation with SARS.
• Dual binding modes of Congo red to amyloid protofibril surface observed in molecular dynamics simulations.
• From the smallest virus to the biggest gene: marching towards gene therapy for duchenne muscular dystrophy.
• In vitro and in vivo evaluation of a novel oral insulin formulation.
• Phenol red interacts with the protofibril-like oligomers of an amyloidogenic hexapeptide NFGAIL through both hydrophobic and aromatic contacts.
• Synthetic intron improves transduction efficiency of trans-splicing adeno-associated viral vectors.
• [A research on healthcare integrating model of medical information system]
• [Application of functional magnetic resonance imaging (fMRI) in the study of visceral hypersensitivity of the gut]
• [Calcium-dependent chloride channels in plasma membrane of oocytes from toad, Bufo bufo gargarizans]
• [Clinical profiles of circulating dendritic cell phenotype and lymphocyte subsets in patients chronically infected with HBV during lamivudine treatment]
• [Clinical value of monitoring CD14+ monocyte human leukocyte antigen (locus) DR levels in the early stage of sepsis]
• [Determination of copper and chromium in the cartridges of permanent marker pen and ink by ICP-AES]
• [Development of an immunochromayography assay method for the detection of Yersinia pestis]
• [Early prenatal diagnosis using fetal cells acquired from different sites of uterine cervix: a preliminary study]
• [Electrocardiograph beat pattern recognition]
• [Expression of CD44 and nm23-H1 protein in the primary and metastatic lymph node lesions in supraglottic and hypopharyngeal cancer]
• [Increasing the speed of construction of the public health incident command system in our country]
• [Induction of growth-associated protein-43 expression in airway afferent nerves of guinea pigs by allergic airway inflammation]
• [Influence of anisodamine on heat-stress in rats]
• [Interatomic distances of molecule for orthohydrogen and parahydrogen]
• [Isolation and identification of Bacillus anthracis in an accidental case]
• [Isolation and identification of Streptococcus suis serotype 2 from sick-pig samples of Sichuan province]
• [Study of sensitivity to impulse noise in guinea pig, rat and mouse]
• [Study on aqueous extraction process for preparation of yuxianling granules]
• [Three types of induced bursting rhythm in rat injured nerves]
• [Zhang Taiyan's Academic Position and Achievements in the Modern History of Chinese Medicine.]