Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles.

Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles. Research Abstract Details 

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Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles. Abstract Text:

E Feitosa,K T Catelam,F A Hasmann,Hans-Olof Johansson,I C Roberto,A Pessoa,E Feitosa,K T Catelam,F A Hasmann,Hans-Olof Johansson,I C Roberto,A Pessoa,

A partial pseudo-ternary phase diagram has been studied for the cethyltrimethylammonium bromide/isooctane:hexanol:butanol/potassium phosphate buffer system, where the two-phase diagram consisting of the reverse micelle phase (L(2)) in equilibrium with the solvent is indicated. Based on these diagrams two-phase systems of reverse micelles were prepared with different compositions of the compounds and used for extraction and recovery of two enzymes, and the percentage of enzyme recovery yield monitored. The enzymes glucose-6-phosphate dehydrogenase (G6PD) and xylose redutase (XR) obtained from Candida guilliermondii yeast were used in the extraction procedures. The recovery yield data indicate that micelles having different composition give selective extraction of enzymes. The method can thus be used to optimize enzyme extraction processes.

Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles. Publishing Authors By Initials

E Feitosa,KT Catelam,FA Hasmann,HO Johansson,IC Roberto,A Pessoa,E Feitosa,KT Catelam,FA Hasmann,HO Johansson,IC Roberto,A Pessoa,

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Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. B, Analytical technolog

VOLUME: 862

Page Numbers: 58-63

Journal Abbreviation: J. Chromatogr. B Analyt. Techn

ISSN: 1570-0232

DAY: 6

MONTH: 11

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 101139554

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Grant and Affiliation Information for Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles.

AFFILIATION: Department of Physics, São Paulo State University, São José do Rio Preto, SP, Brazil.

Country: Netherlands

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MEDLINETA: J Chromatogr B Analyt Technol

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