Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway.

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway. Abstract Text:

Fernando , Canizalez-Roman,Kaitlin E Burlingame,Ken Teter,Jorge E Vidal,

The plasmid-encoded toxin (Pet) of enteroaggregative Escherichia coli is a 104-kDa autotransporter protein that exhibits proteolytic activity against the actin-binding protein alpha-fodrin. Intracellular cleavage of epithelial fodrin by Pet disrupts the actin cytoskeleton, causing both cytotoxic and enterotoxic effects. Intoxication requires the serine protease activity of Pet and toxin endocytosis from clathrin-coated pits. The additional events in the intracellular trafficking of Pet are largely uncharacterized. Here, we determined by confocal microscopy that internalized Pet is transferred from the early endosomes to the Golgi apparatus and then travels to the endoplasmic reticulum (ER). Pet associates with the Sec61p translocon before it moves into the cytosol as an intact, 104-kDa protein. This translocation process contrasts with the export of other ER-translocating toxins, in which only the catalytic A subunit of the AB toxin enters the cytosol. However, like intoxication with these AB toxins, Pet intoxication was inhibited in a subset of mutant CHO cell lines with aberrant activity in the ER-associated degradation pathway of ER-to-cytosol translocation. This is the first report which documents the cell surface-to-ER and ER-to-cytosol trafficking of a bacterial non-AB toxin.

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway. Publishing Authors By Initials

F ,A Canizalez-Roman,KE Burlingame,K Teter,JE Vidal,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

PUBMED ID PMID:

MEDLINE DATE:

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Infection and immunity

VOLUME: 75

Page Numbers: 2101-9

Journal Abbreviation:

ISSN: 0019-9567

DAY: 12

MONTH: 02

YEAR: 2007

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: metabolism

Chemical & Substance for Abstract: Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway. Information

Substance Name: enterotoxin LT

Registry Number: 0

Grant and Affiliation Information for Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway.

AFFILIATION: Department of Cell Biology, Cinvestav-Zacatenco, Ap. Postal 14-740, 07000 México, DF, Mexico. fnavarro@cell.cinvestav.mx

Country: United States

AGENCY: United States NIAID

GRANT: K22 AI054568

ACRONYM: AI

MEDLINETA: Infect Immun

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway Related Publications

• 17-beta-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis.
• Analysis of plant phosphoproteins.
• Application of gas chromatography-triple quadrupole mass spectrometry in the quantification-confirmation of pesticides and polychlorinated biphenyls in eggs at trace levels.
• C. elegans network biology: a beginning.
• Confirmation of organized modularity in the yeast interactome.
• Critical residues at the Ly49 natural killer receptor's homodimer interface determine functional recognition of m157, a mouse cytomegalovirus MHC class I-like protein.
• Effectiveness of MF59-adjuvanted subunit influenza vaccine in preventing hospitalisations for cardiovascular disease, cerebrovascular disease and pneumonia in the elderly.
• Fast determination of herbicides in waters by ultra-performance liquid chromatography/tandem mass spectrometry.
• GLP-1 and changes in glucose tolerance following gastric bypass surgery in morbidly obese subjects.
• Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans.
• Green nanocomposites: synthesis and characterization.
• Influence of a nucleotide oligomerization domain 1 (NOD1) polymorphism and NOD2 mutant alleles on Crohn's disease phenotype.
• Insulin-like growth factor (IGF)-I controls prostate fibromuscular development: IGF-I inhibition prevents both fibromuscular and glandular development in eugonadal mice.
• Laboratorial diagnosis of lymphocytic meningitis.
• Migraine headache: a rare complication after cervicothoracic block.
• Molecular mechanisms of diabetes reversibility after bariatric surgery.
• Network modeling links breast cancer susceptibility and centrosome dysfunction.
• Optical anisotropies in corneal stroma collagen fibers from diabetic spontaneous mice.
• Path-based systems to guide scientists in the maze of biological data sources.
• Pet, a non-AB toxin, is transported and translocated into epithelial cells by a retrograde trafficking pathway.
• Proteomic analysis of secreted exosomes.
• Proteomics analysis of Ring1B/Rnf2 interactors identifies a novel complex with the Fbxl10/Jhdm1B histone demethylase and the Bcl6 interacting corepressor.
• Relationship of clinical and aerobiological pollen data in the north-west of Spain.
• Renal Sodium-Glucose Cotransporter Activity and Aquaporin-2 Expression in Rat Kidney during Chronic Nitric Oxide Synthase Inhibition.
• Reticulocyte-secreted exosomes bind natural IgM antibodies: involvement of a ROS-activatable endosomal phospholipase iPLA2.
• Saccular stimulation of the human cortex: a functional magnetic resonance imaging study.
• Speed of recovery from acute exacerbations of chronic obstructive pulmonary disease after treatment with antimicrobials : results of a two-year study.
• Using a second-order polynomial model to determine the optimum dregs/bark ratio for industrial-biosolid composting: the initial conditioning stage.
• [Post traumatic ocular mycosis due to Penicillium oxalicum.]
• [The Galician woman in Cuba: from exclusion to protection, 1898-1968]