Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision.

Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision. Research Abstract Details 

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Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision. Abstract Text:

A L ,M A Picconi,L V Alonio,D Dalbert,J Mural,O Bartt,G ,G Cervantes,M Lizano,A G ,A ,

OBJECTIVE: The aim of this study was to identify human papillomavirus (HPV) in cervical intraepithelial neoplasia (CIN) lesions and to evaluate the persistence of viral DNA after diathermic large loop excision (DLLE) treatment. STUDY DESIGN: Biopsies from 36 patients with low- and high-grade CIN lesions were studied before and after DLLE treatment looking for HPV sequences. DNA was extracted to perform a radioactive polymerase chain reaction (PCR) using GP 5,6 generic primers. PCR products were analyzed by the single-stranded conformational polymorphism (SSCP) which is a simultaneous detection and typing method. Dot-blot hybridization with generic and type-specific biotinylated oligonucleotide probes was applied in some cases. RESULTS: HPV DNA was found in all pretreatment samples, and the viral type was identified in 80% of them, HPV 16 being the most prevalent. The viral type coincided with that detected in the first biopsy in all except one case. Seventy five percent of the patients (27 cases) were negative for CIN at follow up, but 50% of them remained HPV DNA positive. CONCLUSION: DLLE treatment was effective in removing the CIN lesion but not the HPV. This fact points out the need to asses the presence of HPV in DNA during the follow-up, since viral persistence has been considered a high risk factor for recurrence and/or malignant transformation.

Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision. Publishing Authors By Initials

AL ,MA Picconi,LV Alonio,D Dalbert,J Mural,O Bartt,G ,G Cervantes,M Lizano,AG ,A ,

For similar virus diseases: tumor virus infections research abstracts see: virus diseases: tumor virus infections research

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Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Infectious diseases in obstetrics and gynecology

VOLUME: 6

Page Numbers: 214-9

Journal Abbreviation: Infect Dis Obstet Gynecol

ISSN: 1064-7449

DAY: 21

MONTH: 01

YEAR: 1998

Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision. Information

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LANGUAGE: eng

NlmUniqueID: 9318481

Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision. Keywords Mesh Terms:

KEYWORDS: Tumor Virus Infections

MESH TERMS: virology

Chemical & Substance for Abstract: Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision. Information

Substance Name: DNA, Viral

Registry Number: 0

Grant and Affiliation Information for Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision.

AFFILIATION: Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina. oncovir@sudnet.com.ar

Country: UNITED STATES

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MEDLINETA: Infect Dis Obstet Gynecol

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