Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles.

Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles. Research Abstract Details 

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Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles. Abstract Text:

Denise M Ray,Sherry L Spinelli,Stephen J Pollock,Thomas I Murant,Jamie J O'Brien,Neil Blumberg,Charles W Francis,Mark B Taubman,Richard P Phipps,Denise M Ray,Sherry L Spinelli,Stephen J Pollock,Thomas I Murant,Jamie J O'Brien,Neil Blumberg,Charles W Francis,Mark B Taubman,Richard P Phipps,Denise M Ray,Sherry L Spinelli,Stephen J Pollock,Thomas I Murant,Jamie J O'Brien,Neil Blumberg,Charles W Francis,Mark B Taubman,Richard P Phipps,

Peroxisome proliferator-activated receptor gamma (PPARgamma) and its ligands are important regulators of lipid metabolism, inflammation, and diabetes. We previously demonstrated that anucleate human platelets express the transcription factor PPARgamma and that PPARgamma ligands blunt platelet activation. To further understand the nature of PPARgamma in platelets, we determined the platelet PPARgamma isoform(s) and investigated the fate of PPARgamma following platelet activation. Our studies demonstrated that human platelets contain only the PPARgamma1 isoform and after activation with thrombin, TRAP, ADP or collagen PPARgamma is released from internal stores. PPARgamma release was blocked by a cytoskeleton inhibitor, Latrunculin A. Platelet-released PPARgamma was complexed with the retinoid X receptor (RXR) and retained its ability to bind DNA. Interestingly, the released PPARgamma and RXR were microparticle associated and the released PPARgamma/RXR complex retained DNA-binding ability. Additionally, a monocytic cell line, THP-1, is capable of internalizing PMPs. Further investigation following treatment of these cells with the PPARgamma agonist, rosiglitazone and PMPs revealed a possible transcellular mechanism to attenuate THP-1 activation. These new findings are the first to demonstrate transcription factor release from platelets, revealing the complex spectrum of proteins expressed and expelled from platelets, and suggests that platelet PPARgamma has an undiscovered role in human biology.

Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles. Publishing Authors By Initials

DM Ray,SL Spinelli,SJ Pollock,TI Murant,JJ O'Brien,N Blumberg,CW Francis,MB Taubman,RP Phipps,DM Ray,SL Spinelli,SJ Pollock,TI Murant,JJ O'Brien,N Blumberg,CW Francis,MB Taubman,RP Phipps,DM Ray,SL Spinelli,SJ Pollock,TI Murant,JJ O'Brien,N Blumberg,CW Francis,MB Taubman,RP Phipps,

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Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Thrombosis and haemostasis

VOLUME: 99

Page Numbers: 86-95

Journal Abbreviation: Thromb. Haemost.

ISSN: 0340-6245

DAY: 24

MONTH: Jan

YEAR: 2008

Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles. Information

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LANGUAGE: eng

NlmUniqueID: 7608063

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Grant and Affiliation Information for Peroxisome proliferator-activated receptor gamma and retinoid X receptor transcription factors are released from activated human platelets and shed in microparticles.

AFFILIATION: University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 850, Rochester, New York, USA 14642. E-mail: richard_phipps@urmc.rochester.edu.

Country: Germany

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MEDLINETA: Thromb Haemost

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