Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate.

Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate. Research Abstract Details 

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Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate. Abstract Text:

Thierry ,An X Tran,Jessica V Hankins,Dominique Mengin-Lecreulx,M Stephen Trent,Thierry Touzé,An X Tran,Jessica V Hankins,Dominique Mengin-Lecreulx,M Stephen Trent,

One-third of the lipid A found in the Escherichia coli outer membrane contains an unsubstituted diphosphate unit at position 1 (lipid A 1-diphosphate). We now report an inner membrane enzyme, LpxT (YeiU), which specifically transfers a phosphate group to lipid A, forming the 1-diphosphate species. (32)P-labelled lipid A obtained from lpxT mutants do not produce lipid A 1-diphosphate. In vitro assays with Kdo(2)-[4'-(32)P]lipid A as the acceptor shows that LpxT uses undecaprenyl pyrophosphate as the substrate donor. Inhibition of lipid A 1-diphosphate formation in wild-type bacteria was demonstrated by sequestering undecaprenyl pyrophosphate with the cyclic polypeptide antibiotic bacitracin, providing evidence that undecaprenyl pyrophosphate serves as the donor substrate within whole bacteria. LpxT-catalysed phosphorylation is dependent upon transport of lipid A across the inner membrane by MsbA, a lipid A flippase, indicating a periplasmic active site. In conclusion, we demonstrate a novel pathway in the periplasmic modification of lipid A that is directly linked to the synthesis of undecaprenyl phosphate, an essential carrier lipid required for the synthesis of various bacterial polymers, such as peptidoglycan.

Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate. Publishing Authors By Initials

T ,AX Tran,JV Hankins,D Mengin-Lecreulx,MS Trent,T Touzé,AX Tran,JV Hankins,D Mengin-Lecreulx,MS Trent,

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Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular microbiology

VOLUME: 67

Page Numbers: 264-77

Journal Abbreviation: Mol. Microbiol.

ISSN: 0950-382X

DAY: 27

MONTH: 11

YEAR: 2007

Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Periplasmic phosphorylation of lipid A is linked to the synthesis of undecaprenyl phosphate.

AFFILIATION: Laboratoire des Enveloppes Bactériennes et Antibiotiques, Unité Mixte de Recherche 8619 CNRS, Université Paris-Sud, 91405 Orsay, France.

Country: England

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MEDLINETA: Mol Microbiol

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