Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues.

Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Research Abstract Details 

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Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Abstract Text:

Russell A Norris,Brook Damon,Vladimir Mironov,Vladimir Kasyanov,Anand Ramamurthi,Ricardo Moreno-Rodriguez,Thomas Trusk,Jay D Potts,Richard L Goodwin,Jeff Davis,Stanley Hoffman,Xuejun Wen,Yukiko Sugi,Christine B Kern,Corey H Mjaatvedt,Debi K Turner,Toru Oka,Simon J Conway,Jeffery D Molkentin,Gabor Forgacs,Roger R Markwald,

Periostin is predominantly expressed in collagen-rich fibrous connective tissues that are subjected to constant mechanical stresses including: heart valves, tendons, perichondrium, cornea, and the periodontal ligament (PDL). Based on these data we hypothesize that periostin can regulate collagen I fibrillogenesis and thereby affect the biomechanical properties of connective tissues. Immunoprecipitation and immunogold transmission electron microscopy experiments demonstrate that periostin is capable of directly interacting with collagen I. To analyze the potential role of periostin in collagen I fibrillogenesis, gene targeted mice were generated. Transmission electron microscopy and morphometric analyses demonstrated reduced collagen fibril diameters in skin dermis of periostin knockout mice, an indication of aberrant collagen I fibrillogenesis. In addition, differential scanning calorimetry (DSC) demonstrated a lower collagen denaturing temperature in periostin knockout mice, reflecting a reduced level of collagen cross-linking. Functional biomechanical properties of periostin null skin specimens and atrioventricular (AV) valve explant experiments provided direct evidence of the role that periostin plays in regulating the viscoelastic properties of connective tissues. Collectively, these data demonstrate for the first time that periostin can regulate collagen I fibrillogenesis and thereby serves as an important mediator of the biomechanical properties of fibrous connective tissues.

Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Publishing Authors By Initials

RA Norris,B Damon,V Mironov,V Kasyanov,A Ramamurthi,R Moreno-Rodriguez,T Trusk,JD Potts,RL Goodwin,J Davis,S Hoffman,X Wen,Y Sugi,CB Kern,CH Mjaatvedt,DK Turner,T Oka,SJ Conway,JD Molkentin,G Forgacs,RR Markwald,

For similar integumentary system: skin research abstracts see: integumentary system: skin research

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Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of cellular biochemistry

VOLUME: 101

Page Numbers: 695-711

Journal Abbreviation:

ISSN: 0730-2312

DAY: 1

MONTH: Jun

YEAR: 2007

Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8205768

Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Keywords Mesh Terms:

KEYWORDS: Skin

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues.

AFFILIATION: Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, South Carolina 29425, USA. norrisra@musc.edu

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL66231

ACRONYM: HL

MEDLINETA: J Cell Biochem

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