Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain.

Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. Research Abstract Details 

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Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. Abstract Text:

Sonia Franciosi,Rita De Gasperi,Dara L Dickstein,Daniel F English,Anne B Rocher,William G M Janssen,Daniel Christoffel,Miguel A Gama Sosa,Patrick R Hof,Joseph D Buxbaum,Gregory A Elder,

While the brain vasculature can be imaged with many methods, immunohistochemistry has distinct advantages due to its simplicity and applicability to archival tissue. However, immunohistochemical staining of the murine brain vasculature in aldehyde fixed tissue has proven elusive and inconsistent using current protocols. Here we investigated whether antigen retrieval methods could improve vascular staining in the adult mouse brain. We found that pepsin digestion prior to immunostaining unmasked widespread collagen IV staining of the cerebrovasculature in the adult mouse brain. Pepsin treatment also unmasked widespread vascular staining with laminin, but only marginally improved isolectin B4 staining and did not enhance vascular staining with fibronectin, perlecan or CD146. Collagen IV immunoperoxidase staining was easily combined with cresyl violet counterstaining making it suitable for stereological analyses of both vascular and neuronal parameters in the same tissue section. This method should be widely applicable for labeling the brain vasculature of the mouse in aldehyde fixed tissue from both normal and pathological states.

Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. Publishing Authors By Initials

S Franciosi,R De Gasperi,DL Dickstein,DF English,AB Rocher,WG Janssen,D Christoffel,MA Sosa,PR Hof,JD Buxbaum,GA Elder,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: aspartic endopeptidases: pepsin a research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: aspartic endopeptidases: pepsin a research

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Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of neuroscience methods

VOLUME: 163

Page Numbers: 76-82

Journal Abbreviation:

ISSN: 0165-0270

DAY: 25

MONTH: 02

YEAR: 2007

Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905558

Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. Keywords Mesh Terms:

KEYWORDS: Pepsin A

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain. Information

Substance Name: Pepsin A

Registry Number: EC 3.4.23.1

Grant and Affiliation Information for Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain.

AFFILIATION: Department of Psychiatry, Box 1230, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA.

Country: Netherlands

AGENCY: United States NIA

GRANT: AG020139

ACRONYM: AG

MEDLINETA: J Neurosci Methods

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