PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms.

PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms. Abstract Text:

Juliana Benito,Hao Zheng,Paul E Hardin,

The Drosophila circadian oscillator is composed of autoregulatory period/timeless (per/tim) and Clock (Clk) feedback loops that control rhythmic transcription. In the Clk loop, CLOCK-CYCLE heterodimers activate vrille (vri) and PAR domain protein 1epsilon (Pdp1epsilon) transcription, then sequential repression by VRI and activation by PDP1epsilon mediate rhythms in Clk transcription. Because VRI and PDP1epsilon bind the same regulatory element, the VRI/PDP1epsilon ratio is thought to control the level of Clk transcription. Thus, constant high or low PDP1epsilon levels in clock cells should eliminate Clk mRNA cycling and disrupt circadian oscillator function. Here we show that reducing PDP1epsilon levels in clock cells by approximately 70% via RNA interference or increasing PDP1epsilon levels by approximately 10-fold in clock cells does not alter Clk mRNA cycling or circadian oscillator function. However, constant low or high PDP1epsilon levels in clock cells disrupt locomotor activity rhythms despite persistent circadian oscillator function in brain pacemaker neurons that extend morphologically normal projections into the dorsal brain. These results demonstrate that the VRI/PDP1epsilon ratio neither controls Clk mRNA cycling nor circadian oscillator function and argue that PDP1epsilon is not essential for Clk activation. PDP1epsilon is nevertheless required for behavioral rhythmicity, which suggests that it functions to regulate oscillator output.

PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms. Publishing Authors By Initials

J Benito,H Zheng,PE Hardin,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

PUBMED ID PMID:

MEDLINE DATE:

PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of neuroscience : the official journal

VOLUME: 27

Page Numbers: 2539-47

Journal Abbreviation: J. Neurosci.

ISSN: 1529-2401

DAY: 7

MONTH: Mar

YEAR: 2007

PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8102140

PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: genetics

Chemical & Substance for Abstract: PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms. Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms.

AFFILIATION: Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204, USA.

Country: United States

AGENCY: United States NINDS

GRANT: R01 NS051280-07

ACRONYM: NS

MEDLINETA: J Neurosci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms Related Publications

• A multipurpose, computer-assisted program to improve ambulatory medical care: a preliminary report.
• A review of the management of two common clinical problems found in patients with cystic fibrosis: cystic fibrosis-related diabetes and poor growth.
• Bilateral Wilms tumor in a Wilms tumor family; case report.
• Cardiac disease and sexuality: implications for research and practice.
• Cloprostenol inouced luteal regression in the beef cow. I. Ovarian response and endocrine changes.
• Conditions and trends in the world protein economy.
• Digestion of waste bananas to generate energy in Australia.
• Direct puncture of the proximally occluded internal carotid artery for treatment of carotid cavernous fistulas.
• Efficacy of an HIV prevention program among female adolescents experiencing gender-based violence.
• Embolization of branches arising from the cavernous portion of the internal carotid artery.
• Epidermal morphogenesis.
• Essential and expendable features of the circadian timekeeping mechanism.
• Facial palsy: interpretation of neurologic findings.
• Fluorescence-integrated transmission electron microscopy images: integrating fluorescence microscopy with transmission electron microscopy.
• From spreadsheet to bed sheet.
• Gender and genetic differences in bladder smooth muscle PPAR mRNA in a porcine model of the metabolic syndrome.
• Generalized follicular hamartoma. Complicated by multiple proliferating trichilemmal cysts and palmar pits.
• Insulin resistance in Hispanic large-for-gestational-age neonates at birth.
• Making and breaking contacts: the cellular biology of cadherin regulation.
• Measuring circadian rhythms in olfaction using electroantennograms.
• PDP1epsilon functions downstream of the circadian oscillator to mediate behavioral rhythms.
• PRELIMINARY OBSERVATIONS ON THE REVERSAL OF HYPOVOLEMIA WITH INTRAVENOUS FAT EMULSION. REP 595.
• Postherniorrhaphy groin pain and how to avoid it.
• The missing dimension in developmental biology education.
• The synthesis of histone fractions during the life cycle of mammalian cells. LA-3848-MS.
• Transvenous embolization of dural fistulas involving the transverse and sigmoid sinuses.
• Use of firefly luciferase activity assays to monitor circadian molecular rhythms in vivo and in vitro.
• Vascular metabolic dysfunction and lipotoxicity.
• Volume distribution of normal human erythrocytes and leucocytes. LA-3610-MS.
• rMotifGen: random motif generator for DNA and protein sequences.