[PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene]

[PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene] Research Abstract Details 

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[PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene] Abstract Text:

Xiao-qiang Yang,Bo Jiang,Yong Sun,Ji-de Wang,

OBJECTIVE: To explore a method to rapidly detect the presence of Clostridium difficile. METHODS: PCR was used to amplify DNA fragments from the highly conserved and repeated domains of the 3'-terminal of Clostridium difficile toxin A gene and toxin B genes. RESULTS: The fragments 960 bp and 1,851 bp in length were respectively amplified from toxin A and toxin B, while the control bacteria presented no distinct results. CONCLUSION: The 960 bp and 1,851 bp fragments are specific for Clostridium difficile and PCR can be used to detect Clostridium difficile from the stool. As the peptides encoded by these conserved domains have high hydrophobicity and strong antigenicity, manufacture of the protein vaccine by gene engineering is possible on the basis of these conserved domains.

[PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene] Publishing Authors By Initials

XQ Yang,B Jiang,Y Sun,JD Wang,

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[PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Di 1 jun yi da xue xue bao = Academic journal of t

VOLUME: 22

Page Numbers: 791-3

Journal Abbreviation: Di Yi Jun Yi Da Xue Xue Bao

ISSN: 1000-2588

DAY: 25

MONTH: Sep

YEAR: 2002

[PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene] Information

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LANGUAGE: chi

NlmUniqueID: 9426110

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Grant and Affiliation Information for [PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene]

AFFILIATION: Institute of Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.

Country: China

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MEDLINETA: Di Yi Jun Yi Da Xue Xue Bao

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