PBDE-47-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neurons.

PBDE-47-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neurons. Research Abstract Details 

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PBDE-47-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neurons. Abstract Text:

Ping He,Weihong He,Aiguo Wang,Tao Xia,Bayi Xu,Ming Zhang,Xuemin Chen,

2,2',4,4'-Tetrabromodiphenyl ether (PBDE-47) causes developmental neurotoxicity in animal studies, but the mechanism remains poorly understood. This paper investigates the mechanism by studying the effects of oxidative stress, DNA damage, and apoptosis induced by PBDE-47 in cultured primary rat hippocampal neurons at different PBDE-47-concentrations (0, 2.06, 20.6, and 41.2muM). The results showed that reactive oxygen species (ROS) level, percentage of apoptosis, malondialdehyde (MDA) content, the glutathione peroxidase (GSH-Px) level and the lactic dehydrogenase (LDH) leakage rate were affected by exposure of cells to 41.2muM PDBE-47 (P<0.05), but not to the lower concentrations tested (20.6 and 2.06muM). Reduced glutathione (GSH), superoxide dismutase (SOD), and increased DNA damage (tested by a comet assay) were affected at all concentrations tested in a dose-related manner (P<0.05). These results suggested that PBDE-47 could induce oxidative stress, DNA damage, and apoptosis in primary rat hippocampal neurons. Whether or not this concentration response pattern indicates that ROS leads to DNA damage and/or apoptosis must be confirmed with further experiments.

PBDE-47-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neurons. Publishing Authors By Initials

P He,W He,A Wang,T Xia,B Xu,M Zhang,X Chen,

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PBDE-47-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neurons. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Neurotoxicology

VOLUME: 29

Page Numbers: 124-9

Journal Abbreviation: Neurotoxicology

ISSN: 0161-813X

DAY: 12

MONTH: 10

YEAR: 2007

PBDE-47-induced oxidative stress, DNA damage and apoptosis in primary cultured rat hippocampal neurons. Information

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LANGUAGE: eng

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AFFILIATION: Department of Environmental Health and MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, People's Republic of China.

Country: Netherlands

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MEDLINETA: Neurotoxicology

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