Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites.

Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites. Research Abstract Details 

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Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites. Abstract Text:

Andrey Efimov,Natalia Schiefermeier,Ilya Grigoriev,Michael C Brown,Christopher E Turner,J Victor Small,Irina Kaverina,

An organized microtubule array is essential for the polarized motility of fibroblasts. Dynamic microtubules closely interact with focal adhesion sites in migrating cells. Here, we examined the effect of focal adhesions on microtubule dynamics. We observed that the probability of microtubule catastrophes (transitions from growth to shrinkage) was seven times higher at focal adhesions than elsewhere. Analysis of the dependence between the microtubule growth rate and catastrophe probability throughout the cytoplasm revealed that a nonspecific (mechanical or spatial) factor provided a minor contribution to the catastrophe induction by decreasing microtubule growth rate at adhesions. Strikingly, at the same growth rate, the probability of catastrophes was significantly higher at adhesions than elsewhere, indicative of a site-specific biochemical trigger. The observed catastrophe induction occurred at adhesion domains containing the scaffolding protein paxillin that has been shown previously to interact with tubulin. Furthermore, replacement of full-length paxillin at adhesion sites by microinjected paxillin LIM2-LIM3 domains suppressed microtubule catastrophes exclusively at adhesions. We suggest that paxillin influences microtubule dynamics at focal adhesions by serving as a scaffold for a putative catastrophe factor and/or regulating its exposure to microtubules.

Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites. Publishing Authors By Initials

A Efimov,N Schiefermeier,I Grigoriev,MC Brown,CE Turner,JV Small,I Kaverina,

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Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of cell science

VOLUME: 121

Page Numbers: 196-204

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 15

MONTH: Jan

YEAR: 2008

Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites. Information

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LANGUAGE: eng

NlmUniqueID: 52457

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Grant and Affiliation Information for Paxillin-dependent stimulation of microtubule catastrophes at focal adhesion sites.

AFFILIATION: Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN, USA.

Country: England

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MEDLINETA: J Cell Sci

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