Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia.

Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. Abstract Text:

Doyeob Kim,Min Wang,Qi Cai,Heddwen Brooks,Gregory R Dressler,Doyeob Kim,Min Wang,Qi Cai,Heddwen Brooks,Gregory R Dressler,

Pax transactivation-domain interacting protein (PTIP) is a widely expressed nuclear protein that is essential for early embryonic development. PTIP was first identified on the basis of its interactions with the developmental regulator Pax2 but can also bind to other nuclear transcription factors. The Pax2 protein is essential for development of the renal epithelia and for regulating the response of mature collecting ducts to hyperosmotic stress. For determination of whether PTIP also functions in more differentiated cell types, the Cre-LoxP system was used to delete the ptip gene in the renal collecting ducts using Ksp-Cre driver mice. Collecting duct-specific ptip knockout mice were viable with little discernible phenotype under normal physiologic conditions. However, collecting duct-specific ptip mutants were unable to concentrate urine after the treatment of desamino-cis, D-arginine vasopressin, an antidiuretic hormone. Furthermore, aquaporin-2 (AQP2) expression in the inner medulla of the ptip knockout mice was decreased approximately 10-fold compared with that of wild-type littermates. Expression level of tonicity responsive enhancer binding protein, a transcription factor of AQP2, is not altered in the mutant mice, but its nuclear localization in the inner medulla is unresponsive after treatment with vasopressin agonists. This was due, at least in part, to decreased expression of the arginine vasopressin receptor 2 in ptip mutants. Furthermore, ptip null inner medullary collecting duct cells were sensitive to hyperosmolality in vitro. Thus, ptip is required for the urine concentration mechanism by modulating arginine vasopressin receptor 2 and AQP2 expression in the inner medulla. The data suggest an essential role for ptip in regulating urine concentration and in controlling survival of collecting duct epithelial cells in high osmolality.

Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. Publishing Authors By Initials

D Kim,M Wang,Q Cai,H Brooks,GR Dressler,D Kim,M Wang,Q Cai,H Brooks,GR Dressler,

For similar proteins: membrane proteins: receptors, cell surface: receptors, g-protein-coupled: receptors, vasopressin research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, g-protein-coupled: receptors, vasopressin research

PUBMED ID PMID:

MEDLINE DATE:

Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Society of Nephrology : JA

VOLUME: 18

Page Numbers: 1458-65

Journal Abbreviation: J. Am. Soc. Nephrol.

ISSN: 1046-6673

DAY: 11

MONTH: 04

YEAR: 2007

Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9013836

Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. Keywords Mesh Terms:

KEYWORDS: Receptors, Vasopressin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia. Information

Substance Name: Receptors, Vasopressin

Registry Number: 0

Grant and Affiliation Information for Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia.

AFFILIATION: Department of Pathology, University of Michigan, Ann Arbor, Michigan, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: R01 DK073722-02

ACRONYM: DK

MEDLINETA: J Am Soc Nephrol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia Related Publications

• A comparative study of five fatal cases of Taxus poisoning.
• A new strategy on pitch estimation in polyphonic musical audio.
• Another niche for Notch.
• Botulinum toxin drugs: future developments.
• Botulinum toxin treatment of axial and cervical dystonia.
• Centrifugation of rickettsiae and viruses onto cells and its effect on infection.
• Cerebral air embolism associated with spontaneous pneumothorax.
• DNA flow cytometry measurements and their clinical relevance in node-negative breast cancer patients.
• Digit ratio (2D:4D) in Lithuania once and now: testing for sex differences, relations with eye and hair color, and a possible secular change.
• Molecular subtypes of breast cancer in relation to paclitaxel response and outcomes in women with metastatic disease: results from CALGB 9342.
• Osteopathic education looks ahead.
• PTEN modulates GDNF/RET mediated chemotaxis and branching morphogenesis in the developing kidney.
• Pax transactivation-domain interacting protein is required for urine concentration and osmotolerance in collecting duct epithelia.
• Percussion of the sternum. I. Aid to differentiation of pericardial effusion and cardiac dilatation.
• Phenotypic ranges and relationships among carcass and meat palatability traits for fourteen cattle breeds, and heritabilities and expected progeny differences for Warner-Bratzler shear force in three beef cattle breeds.
• The BRCT-domain containing protein PTIP links PAX2 to a histone H3, lysine 4 methyltransferase complex.
• The cellular basis of kidney development.
• Topical application of mafenide acetate. Its association with erythema multiforme and cutaneous reactions.
• [An improved model of a catheterised human bladder for screening bactericidal agents.]
• [Anal pain.]
• [Categories of kinetic visual acuity in a study group; the characteristics of the curves]
• [Current outlook on botulinum toxin medications]
• [Essay of physio-pathology of graded tubage. I. Participation of the duodenum in certain anomalies and, especially, of Oddi's closure time and in time of bile.]
• [Hemorrhagic macular choroidopathy in young adults; the problems of diagnosis and treatment]
• [Kinetic visual acuity (KVA) as a function of the form and color of the visual tests employed]
• [Kinetic visual acuity (KVA)]
• [Kinetic visual acuity as a function of age, fatigue, training and natural selection of female textile workers at various work stations]
• [Kinetic visual acuity as a means of functional examination in ophthalmology]
• [Spontaneous ciliochoroidal detachment (a case report)]
• [Systematic investigation of digestive tract tumors by a scaled clinical pre-investigation technic.]