Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation.

Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation. Research Abstract Details 

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Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation. Abstract Text:

Elisabeth S Feary,Jennifer L Juengel,Peter Smith,Michelle C French,Anne R O'Connell,Stephen B Lawrence,Susan M Galloway,George H Davis,Kenneth P McNatty,Elisabeth S Feary,Jennifer L Juengel,Peter Smith,Michelle C French,Anne R O'Connell,Stephen B Lawrence,Susan M Galloway,George H Davis,Kenneth P McNatty,Elisabeth S Feary,Jennifer L Juengel,Peter Smith,Michelle C French,Anne R O'Connell,Stephen B Lawrence,Susan M Galloway,George H Davis,Kenneth P McNatty,

Woodlands sheep have a putative genetic mutation (FecX2(W)) that increases ovulation rate. At present, the identity of FecX2(W) is unknown. The trait does not appear to be due to the previously described mutations in bone morphogenetic protein 15 (BMP15), growth differentiation factor 9 (GDF9), or bone morphogenetic protein receptor type 1B (BMPR1B) that affect ovulation rate in sheep. Potentially, FecX2(W) could be an unidentified genetic mutation in BMP15 or in the closely related GDF9, which interacts with BMP15 to control ovarian function. Alternatively, FecX2(W) may affect ovulation rate by changing the expression patterns in the molecular pathways activated by genes known to regulate ovulation rate. The objectives of these experiments were to sequence the complete coding region of the BMP15 and GDF9 genes, determine the patterns of expression of mRNAs encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 during follicular development, and characterize the follicular populations in ewes heterozygous for the Woodlands mutation and their wild-type contemporaries. No differences in the coding sequences of BMP15 or GDF9 genes were identified that were associated with enhanced ovulation rate. The expression patterns of GDF9 and BMPR2 mRNAs were not different between genotypes. However, expression of BMP15 mRNA was less in oocytes of FecX2(W) ewes in large preantral and antral follicles. Expression of ALK5 mRNA was significantly higher in the oocytes of FecX2(W) ewes, whereas expression of BMPR1B was decreased in both oocytes and granulosa cells of FecX2(W) ewes. FecX2(W) ewes also had increased numbers of antral follicles <1 mm in diameter. These follicles were smaller in average diameter, with the oocytes also being of a smaller mean diameter. Given that a mutation in BMP15 or BMPR1B results in increased ovulation rates in sheep, the differences in expression levels of BMP15 and BMPR1B may play a role in the increase in ovulation rate observed in Woodlands ewes with the FecX2(W) mutation.

Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation. Publishing Authors By Initials

ES Feary,JL Juengel,P Smith,MC French,AR O'Connell,SB Lawrence,SM Galloway,GH Davis,KP McNatty,ES Feary,JL Juengel,P Smith,MC French,AR O'Connell,SB Lawrence,SM Galloway,GH Davis,KP McNatty,ES Feary,JL Juengel,P Smith,MC French,AR O'Connell,SB Lawrence,SM Galloway,GH Davis,KP McNatty,

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Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biology of reproduction

VOLUME: 77

Page Numbers: 990-8

Journal Abbreviation: Biol. Reprod.

ISSN: 0006-3363

DAY: 22

MONTH: 08

YEAR: 2007

Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation. Information

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LANGUAGE: eng

NlmUniqueID: 207224

Patterns of Expression of Messenger RNAs Encoding GDF9, BMP15, TGFBR1, BMPR1B, and BMPR2 During Follicular Development and Characterization of Ovarian Follicular Populations in Ewes Carrying the Woodlands FecX2W Mutation. Keywords Mesh Terms:

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AFFILIATION: Wallaceville Animal Research Centre, AgResearch, Upper Hutt 5140, New Zealand.

Country: United States

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MEDLINETA: Biol Reprod

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