Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells.

Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells. Abstract Text:

Sandra S Wise,Amie L Holmes,John Pierce Wise,

Particulate hexavalent chromium (Cr(VI)) is a well-established human lung carcinogen. It is currently a major public health concern, there is widespread exposure to it in occupational settings and to the general public. However, despite the potential widespread exposure and the fact that the lung is its target organ, few studies have considered the toxic effects of particulate Cr(VI) in human lung cells. Accordingly, we used lead chromate as a model particulate Cr(VI) compound and determined its cytotoxicity and genotoxicity in cultured human bronchial epithelial cells, using BEP2D cells as a model cell line. We found that lead chromate induced concentration-dependent cytotoxicity in BEP2D cells after a 24h exposure. Specifically, the relative survival was 78, 59, 53, 46 and 0% after exposure to 0.5, 1, 5, 10 and 50 microg/cm(2) lead chromate, respectively. Similarly, the amount of chromosome damage increased with concentration after 24h exposure to lead chromate. Specifically, 0.5, 1, 5 and 10 microg/cm(2) damaged 10, 13, 20 and 28% of metaphase cells with the total amount of damage reaching 11, 15, 24 and 36 aberrations per 100 metaphases, respectively. Lead chromate (50 microg/cm(2) lead chromate) induced profound cell cycle delay and no metaphases were found. In addition we investigated the effects of soluble hexavalent chromium, sodium chromate, in this cell line. We found that 1, 2.5, 5 and 10 microM sodium chromate induced 66, 35, 0 and 0% relative survival, respectively. The amount of chromosome damage increased with concentration after 24h exposure to sodium chromate. Specifically, 1, 2.5 and 5 microM damaged 25, 34 and 41% of metaphase cells with the total amount of damage reaching 33, 59 and 70 aberrations per 100 metaphases, respectively. Ten micromolar sodium chromate induced profound cell cycle delay and no metaphases were found. Overall the data clearly indicate that hexavalent Cr(VI) is cytotoxic and genotoxic to human lung epithelial cells.

Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells. Publishing Authors By Initials

SS Wise,AL Holmes,JP Wise,

For similar natural sciences: chemistry: chemistry, physical: solubility research abstracts see: natural sciences: chemistry: chemistry, physical: solubility research

PUBMED ID PMID:

MEDLINE DATE:

Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Mutation research

VOLUME: 610

Page Numbers: 2-7

Journal Abbreviation: Mutat. Res.

ISSN: 0027-5107

DAY: 26

MONTH: 07

YEAR: 2006

Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 400763

Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells. Keywords Mesh Terms:

KEYWORDS: Solubility

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells. Information

Substance Name: sodium chromate(VI)

Registry Number: 7775-11-3

Grant and Affiliation Information for Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells.

AFFILIATION: Wise Laboratory of Environmental and Genetic Toxicology, Maine Center for Toxicology and Environmental Health, University of Southern Maine, PO Box 9300, Portland, ME 04103-9300, United States.

Country: Netherlands

AGENCY: United States NIEHS

GRANT: ES10838

ACRONYM: ES

MEDLINETA: Mutat Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells Related Publications

• A low-profile three-dimensional silicon/parylene stimulating electrode array for neural prosthesis applications.
• All-normal-dispersion femtosecond fiber laser with pulse energy above 20 nJ.
• Atomoxetine pharmacokinetics in healthy Chinese subjects and effect of the CYP2D6*10 allele.
• Benzo[a]pyrene cytotoxicity in right whale (Eubalaena glacialis) skin, testis and lung cell lines.
• Bladder and upper tract urothelial cancer.
• Chronic exposure to particulate chromate induces spindle assembly checkpoint bypass in human lung cells.
• Chronological gene expression of parathyroid hormone-related protein (PTHrP) in the stellate reticulum of the rat: implications for tooth eruption.
• Combined Training and beta-Alanine Supplementation Muscle Carnosine Synthesis, Ventilatory Threshold and Exercise Capacity in Cyclists: 2069: Board #139 May 31 3:30 PM 5:00 PM.
• Commentary: "will medical solutions to sexual problems make sexological care and science obsolete?".
• Concentrations in plasma, epithelial lining fluid, alveolar macrophages and bronchial mucosa after a single intravenous dose of 1.6 mg/kg of iclaprim (AR-100) in healthy men.
• Determination of HBCD, PBDEs and MeO-BDEs in California sea lions (Zalophus californianus) stranded between 1993 and 2003.
• Effect of vascular endothelial growth factor on RANK gene expression in osteoclast precursors and on osteoclastogenesis.
• Efficient high-energy femtosecond pulse compression in quadratic media with flattop beams.
• Estradiol targets synaptic proteins to induce glutamatergic synapse formation in cultured hippocampal neurons: critical role of estrogen receptor-alpha.
• Feasibility of retinoids for the treatment of emphysema study.
• Four week supplementation with mixed fruit and vegetable juice concentrates increased protective serum antioxidants and folate and decreased plasma homocysteine in Japanese subjects.
• Kidney cancer.
• Ku80 deficiency does not affect particulate chromate-induced chromosome damage and cytotoxicity in Chinese hamster ovary cells.
• Male urethral stricture disease.
• Neural correlates of intelligibility in speech investigated with noise vocoded speech--a positron emission tomography study.
• Nonlocal explanation of stationary and nonstationary regimes in cascaded soliton pulse compression.
• Particulate and soluble hexavalent chromium are cytotoxic and genotoxic to human lung epithelial cells.
• Role of the Fancg gene in protecting cells from particulate chromate-induced chromosome instability.
• Scaling of femtosecond Yb-doped fiber amplifiers to tens of microjoule pulse energy via nonlinear chirped pulse amplification.
• Sex differences in severe pulmonary emphysema.
• Standard reference materials for foods and dietary supplements.
• The clastogenic effects of chronic exposure to particulate and soluble Cr(VI) in human lung cells.
• The continuing conundrum of the LEA proteins.
• The shift from defined benefit pensions to 401(k) plans and the pension assets of the baby boom cohort.
• XRCC1 protects cells from chromate-induced chromosome damage, but does not affect cytotoxicity.