Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA.

Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA. Research Abstract Details 

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Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA. Abstract Text:

Kamalendra Singh,Aashish Srivastava,Smita S Patel,Mukund J Modak,

The replication of the genome requires the removal of RNA primers from the Okazaki fragments and their replacement by DNA. In prokaryotes, this process is completed by DNA polymerase I by means of strand displacement DNA synthesis and 5 '-nuclease activity. Here, we demonstrate that the strand displacement DNA synthesis is facilitated by the collective participation of Ser(769), Phe(771), and Arg(841) present in the fingers subdomain of DNA polymerase I. The steady and presteady state kinetic analysis of the properties of appropriate mutant enzymes suggest that: (a) Ser(769) and Phe(771) together are involved in the strand separation via the formation of a flap structure, and (b) Arg(841) interacts with the template strand to achieve the optimal strand separation and DNA synthesis. The amino acid residues Ser(769) and Phe(771) are constituents of the O1-helix, which together with O and O2 helices form a 3-helix bundle structure. We note that this 3-helix bundle motif also exists in prokaryotic RNA polymerase. Thus in both DNA and RNA polymerases, this motif may have been adopted to achieve the strand separation function.

Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA. Publishing Authors By Initials

K Singh,A Srivastava,SS Patel,MJ Modak,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

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Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 10594-604

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 26

MONTH: 01

YEAR: 2007

Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA. Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: metabolism

Chemical & Substance for Abstract: Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for Participation of the fingers subdomain of Escherichia coli DNA polymerase I in the strand displacement synthesis of DNA.

AFFILIATION: Department of Biochemistry and Molecular Biology, University of Medicine and Dentistry-New Jersey Medical School, Newark, New Jersey 07103, USA. ksingh@umdnj.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM55310

ACRONYM: GM

MEDLINETA: J Biol Chem

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