Partial purification and properties of phospholipase A2 from rat liver mitochondria.

Partial purification and properties of phospholipase A2 from rat liver mitochondria. Research Abstract Details 

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Partial purification and properties of phospholipase A2 from rat liver mitochondria. Abstract Text:

Y Natori,K Karasawa,H Arai,Y Tamori-Natori,S Nojima,

Phospholipase A2 of rat liver mitochondria was purified approximately 1,400-fold by extraction with KCl, and chromatographies on a Sephadex G-75 column and a diacyl-glycerophosphocholine-Sepharose affinity column. The purified enzyme was very labile when incubated either at 37 degrees C or 0 degrees C, and lost its activity within a few hours. Phospholipids or detergents in the solution protected the enzyme against inactivation. The purified phospholipase A2 preferentially hydrolyzed phosphatidylethanolamine, especially if it contained linoleic acid. The enzyme showed low activity for phosphatidylcholine or phosphatidylinositol.

Partial purification and properties of phospholipase A2 from rat liver mitochondria. Publishing Authors By Initials

Y Natori,K Karasawa,H Arai,Y Tamori-Natori,S Nojima,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

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Partial purification and properties of phospholipase A2 from rat liver mitochondria. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 93

Page Numbers: 631-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1983

Partial purification and properties of phospholipase A2 from rat liver mitochondria. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Partial purification and properties of phospholipase A2 from rat liver mitochondria. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: Partial purification and properties of phospholipase A2 from rat liver mitochondria. Information

Substance Name: Phospholipases A2

Registry Number: EC 3.1.1.4

Grant and Affiliation Information for Partial purification and properties of phospholipase A2 from rat liver mitochondria.

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Country: JAPAN

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MEDLINETA: J Biochem

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