Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis.

Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis. Research Abstract Details 

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Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis. Abstract Text:

Tohru Hayakawa,Rie Kanagawa,Yosuke Kotani,Mayumi Kimura,Masashi Yamagiwa,Yoshiharu Yamane,So Takebe,Hiroshi Sakai,Tohru Hayakawa,Rie Kanagawa,Yosuke Kotani,Mayumi Kimura,Masashi Yamagiwa,Yoshiharu Yamane,So Takebe,Hiroshi Sakai,

A novel crystal protein that exhibited potent cytotoxicity against human leukemic T-cells was cloned from the Bacillus thuringiensis TK-E6 strain. The protein, designated as parasporin-2Ab (PS2Ab), was a polypeptide of 304 amino acid residues with a predicted molecular weight of 33,017. The deduced amino acid sequence of PS2Ab showed significant homology (84% identitiy) to parasporin-2Aa (PS2Aa) from the B. thuringiensis A1547 strain. Upon processing of PS2Ab with proteinase K, the active form of 29 kDa was produced. The activated PS2Ab showed potent cytotoxicity against MOLT-4 and Jurkat cells and the EC(50) values were estimated as 0.545 and 0.745 ng/mL, respectively. The cytotoxicity of PS2Ab was significantly higher than that of PS2Aa reported elsewhere. Although both cytotoxins were structurally related, it was thought that the minor differences found were responsible for the different cytotoxicities of PS2Ab and PS2Aa.

Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis. Publishing Authors By Initials

T Hayakawa,R Kanagawa,Y Kotani,M Kimura,M Yamagiwa,Y Yamane,S Takebe,H Sakai,T Hayakawa,R Kanagawa,Y Kotani,M Kimura,M Yamagiwa,Y Yamane,S Takebe,H Sakai,

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Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Current microbiology

VOLUME: 55

Page Numbers: 278-83

Journal Abbreviation: Curr. Microbiol.

ISSN: 0343-8651

DAY: 13

MONTH: 08

YEAR: 2007

Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis. Information

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LANGUAGE: eng

NlmUniqueID: 7808448

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Grant and Affiliation Information for Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis.

AFFILIATION: Graduate School of Natural Science and Technology, Okayama University, Tsushima-Naka 3-1-1, Okayama, Japan.

Country: United States

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MEDLINETA: Curr Microbiol

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