Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master.

Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master. Research Abstract Details 

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Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master. Abstract Text:

Joohoon Kim,Richard M Crooks,Joohoon Kim,Richard M Crooks,Joohoon Kim,Richard M Crooks,

A new method for fabrication of RNA microarrays is described. The approach involves cohybridization of a short, biotinylated DNA oligonucleotide and an RNA probe sequence to DNA templates spotted onto a master array. Next, the short DNA sequence and the RNA probe are linked using a T4 DNA ligase. Finally, a poly(dimethylsiloxane) (PDMS) monolith modified on the surface with streptavidin is brought into conformal contact with the master array. This results in binding of the biotinylated DNA/RNA oligonucleotides to the PDMS surface. When the two substrates are mechanically separated, the DNA/RNA oligonucleotides transfer to the PDMS replica, and the DNA oligonucleotides remaining on the master array are ready to template another RNA replica array. This sequence can be repeated for at least 18 cycles using a single master array. RNA arrays consisting of up to three different oligonucleotide sequences and consisting of up to 2500 individual approximately 70 mum spots have been prepared.

Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master. Publishing Authors By Initials

J Kim,RM Crooks,J Kim,RM Crooks,J Kim,RM Crooks,

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Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 8994-9

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 27

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 370536

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Grant and Affiliation Information for Parallel fabrication of RNA microarrays by mechanical transfer from a DNA master.

AFFILIATION: Department of Chemistry and Biochemistry, Texas Materials Institute, Center for Nano and Molecular Science and Technology, The University of Texas at Austin, 1 University Station, A5300, Austin, Texas 78712-0165.

Country: United States

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MEDLINETA: Anal Chem

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