Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations.

Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. Abstract Text:

Johann Elbaz,Bella Shlyahovsky,Di Li,Itamar Willner,

A bifunctional aptamer that includes two aptamer units for cocaine and adenosine 5'-monophosphate (AMP) is blocked by a nucleic acid to form a hybrid structure with two duplex regions. The blocked bifunctional aptamer assembly is used as a functional structure for the simultaneous sensing of cocaine or AMP. The blocked bifunctional aptamer is dissociated by either of the two analytes, and the readout of the separation of the sensing structure is accomplished by a colorimetric detection, by a released DNAzyme, or by electronic means that use Faradaic impedance spectroscopy or field-effect transistors. In one configuration, the blocked bifunctional aptamer structure is separated by the substrates cocaine or AMP, and the displaced blocker units act as a horseradish peroxidase-mimicking DNAzyme that permits the colorimetric detection of the analytes. In the second system, the blocked bifunctional aptamer hybrid is associated with a Au electrode. The displacement of the aptamer by any of the substrates alters the interfacial electron transfer resistance at the electrode surface, thus providing an electronic signal for the sensing process. In the third configuration, the blocked aptamer hybrid is linked to the gate of a field-effect transistor device. The separation of the complex by means of any of the analytes, cocaine, or AMP alters the gate potential, and this allows the electronic transduction of the sensing process by following the changes in the gate-to-source potentials. The different systems enable not only the simultaneous detection of the two analytes, but they provide a functional assembly that performs a logic gate "OR" operation.

Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. Publishing Authors By Initials

J Elbaz,B Shlyahovsky,D Li,I Willner,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chembiochem : a European journal of chemical biolo

VOLUME: 9

Page Numbers: 232-9

Journal Abbreviation: Chembiochem

ISSN: 1439-7633

DAY: 25

MONTH: Jan

YEAR: 2008

Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100937360

Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations.

AFFILIATION: Institute of Chemistry, Farkas Center for Light-Induced Processes, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Chembiochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations Related Publications

• A DNAzyme cascade for the amplified detection of Pb(2+) ions or l-histidine.
• A comparative study of spondylolisthesis in operations on adolescents and adults.
• A polycatenated DNA scaffold for the one-step assembly of hierarchical nanostructures.
• Acetylcholine esterase-labeled CdS nanoparticles on electrodes: photoelectrochemical sensing of the enzyme inhibitors.
• Amplified DNA sensing and immunosensing by the rotation of functional magnetic particles.
• Amplified electrochemical detection of DNA through the aggregation of au nanoparticles on electrodes and the incorporation of methylene blue into the DNA-crosslinked structure.
• Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin.
• Catalytic beacons for the detection of DNA and telomerase activity.
• Clarification of the memory artefact in the assessment of suggestibility.
• Electrochemical control of surface properties using a quinone-functionalized monolayer: effects of donor-acceptor complexes.
• Fluorescence detection of DNA by the catalytic activation of an aptamer/thrombin complex.
• Following a protein kinase activity using a field-effect transistor device.
• Individual psychotherapy with the depressed elderly outpatient: an overview.
• Infrared Spectrum of Matrix Isolated Selenoformaldehyde, CH2Se, and CD2Se.
• Lighting-up the dynamics of telomerization and DNA replication by CdSe-ZnS quantum dots.
• Magneto-stimulated hydrodynamic control of electrocatalytic and bioelectrocatalytic processes.
• Moiré topography--a method for school screening of scoliosis.
• Negro tuberculosis control.
• Optical detection of glucose and acetylcholine esterase inhibitors by H2O2-sensitive CdSe/ZnS quantum dots.
• Parallel analysis of two analytes in solutions or on surfaces by using a bifunctional aptamer: applications for biosensing and logic gate operations.
• Photoelectrochemical and optical applications of semiconductor quantum dots for bioanalysis.
• Photosensitivity-enabled dispersion controllability for quasi-phase-matching in photonic crystal fibers.
• Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA.
• Spectrally efficient slow light using multilevel phase-modulated formats.
• Treatment of Postcraniotomy Tension Pneumocephalus via Endoscopic Closure of the Nasofrontal Recess.
• Trifluoromethylboranes and -borates: new synthetic strategies and applications.
• [Areas of tension between dental office and laboratory]
• [Gnathomat--possibilities and facts from the viewpoint of the laboratory]
• [The gnathomat and its use]
• [What are we waiting for?]