Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library.

Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library. Research Abstract Details 

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Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library. Abstract Text:

M Nakamura,H Watanabe,Y Nishimiya,K Tsumoto,K Ishimura,I Kumagai,

We have established a method for selecting binding phages from a phage immunoglobulin heavy chain variable region (VH) library by panning with nitrocellulose membranes (membrane panning). To evaluate the concentrating ability of membrane panning for binding phages, a phage VH library containing clones that bind to hen egg white lysozyme (HEL) was used for panning against HEL. The efficiency of our method was as high as that of panning with magnetic beads. In addition, we performed membrane panning against target proteins and isolated the binding phages. The human VH genes of these phages were cloned and expressed as VH-bacterial alkaline phosphatase (PhoA) conjugates (VH-PhoA) in Escherichia coli. The dose-dependent binding of VH-PhoA to target proteins was confirmed by dot blotting. When applied to disease-associated antibodies, these methods will likely benefit clinical research. In addition, these techniques may be applicable to systematic analysis in proteome studies.

Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library. Publishing Authors By Initials

M Nakamura,H Watanabe,Y Nishimiya,K Tsumoto,K Ishimura,I Kumagai,

For similar peptides: peptide library research abstracts see: peptides: peptide library research

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Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 129

Page Numbers: 209-12

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 2001

Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library. Keywords Mesh Terms:

KEYWORDS: Peptide Library

MESH TERMS: immunology

Chemical & Substance for Abstract: Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library. Information

Substance Name: Muramidase

Registry Number: EC 3.2.1.17

Grant and Affiliation Information for Panning of a phage VH library using nitrocellulose membranes: application to selection of a human VH library.

AFFILIATION: Department of Anatomy and Cell Biology, School of Medicine, Tokushima University, 3-18-15 Kuramoto, Tokushima 770-8503, Japan.

Country: Japan

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MEDLINETA: J Biochem

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