PALS1 regulates E-cadherin trafficking in mammalian epithelial cells.

PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Research Abstract Details 

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PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Abstract Text:

Qian Wang,Xiao-Wei Chen,Ben Margolis,

Protein Associated with Lin Seven 1 (PALS1) is an evolutionarily conserved scaffold protein that targets to the tight junction in mammalian epithelia. Prior work in our laboratory demonstrated that the knockdown of PALS1 in Madin Darby canine kidney cells leads to tight junction and polarity defects. We have created new PALS1 stable knockdown cell lines with more profound reduction of PALS1 expression, and a more severe defect in tight junction formation was observed. Unexpectedly, we also observed a severe adherens junction defect, and both defects were corrected when PALS1 wild type and certain PALS1 mutants were expressed in the knockdown cells. We found that the adherens junction structural component E-cadherin was not effectively delivered to the cell surface in the PALS1 knockdown cells, and E-cadherin puncta accumulated in the cell periphery. The exocyst complex was also found to be mislocalized in PALS1 knockdown cells, potentially explaining why E-cadherin trafficking is disrupted. Our results suggest a broad and evolutionarily conserved role for the tight junction protein PALS1 in the biogenesis of adherens junction.

PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Publishing Authors By Initials

Q Wang,XW Chen,B Margolis,

For similar cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research abstracts see: cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research

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PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 874-85

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 20

MONTH: 12

YEAR: 2006

PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Information

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LANGUAGE: eng

NlmUniqueID: 9201390

PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Keywords Mesh Terms:

KEYWORDS: Tight Junctions

MESH TERMS: metabolism

Chemical & Substance for Abstract: PALS1 regulates E-cadherin trafficking in mammalian epithelial cells. Information

Substance Name: Mutant Proteins

Registry Number: 0

Grant and Affiliation Information for PALS1 regulates E-cadherin trafficking in mammalian epithelial cells.

AFFILIATION: Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: P60 DK20572

ACRONYM: DK

MEDLINETA: Mol Biol Cell

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