p53 mutations in human head and neck cancer cell lines.

p53 mutations in human head and neck cancer cell lines. Research Abstract Details 

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p53 mutations in human head and neck cancer cell lines. Abstract Text:

A Kiuru,K Servomaa,R Grénman,J Pulkkinen,T Rytömaa,

The p53 tumour suppressor gene is commonly mutated in human cancers. We performed a molecular analysis of the frequency and spectrum of p53 gene mutations in 40 cell lines (23 from oral cavity tumours and 17 from larynx tumours) derived from 33 patients with squamous cell carcinoma of the head and neck (SCCHN). Using PCR, SSCP, and sequence analysis, we detected the mutated p53 gene in 26 patients (79%); in 23 patients (70%) the wild-type allele of the p53 gene was deleted. Four patients had 2 p53 gene mutations each, and thus the total number of p53 mutations observed was 30. Seven patients had 2 cell lines each, established from the primary and recurrent/metastatic tumour, and the status of the p53 gene (mutant or normal) was identical in both cell lines. Forty percent of the mutations were transitions, 33% transversions, and 27% deletions, insertions and other more complicated changes. In oral cavity tumours the predominant mutation type was G:C-->A:T transition at a CpG site (50% of mutations), and in larynx tumours the predominant type was G:C-->T:A transversion (50% of mutations). These suggest endogenous and exogenous factors in tumour etiology. The G:C-->T:A transversions in larynx tumours are probably associated with mutagenic components in the cigarette smoke, but the causative factor in G:C-->A:T transitions (apparent oxidative damage) remains to be identified.

p53 mutations in human head and neck cancer cell lines. Publishing Authors By Initials

A Kiuru,K Servomaa,R Grénman,J Pulkkinen,T Rytömaa,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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p53 mutations in human head and neck cancer cell lines. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta oto-laryngologica. Supplementum

VOLUME: 529

Page Numbers: 237-40

Journal Abbreviation:

ISSN: 0365-5237

DAY: 13

MONTH: 02

YEAR: 1997

p53 mutations in human head and neck cancer cell lines. Information

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LANGUAGE: eng

NlmUniqueID: 370355

p53 mutations in human head and neck cancer cell lines. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: genetics

Chemical & Substance for Abstract: p53 mutations in human head and neck cancer cell lines. Information

Substance Name: DNA, Neoplasm

Registry Number: 0

Grant and Affiliation Information for p53 mutations in human head and neck cancer cell lines.

AFFILIATION: Finnish Centre for Radiation and Nuclear Safety, Helsinki, Finland.

Country: NORWAY

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MEDLINETA: Acta Otolaryngol Suppl

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