p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity.

p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity. Research Abstract Details 

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p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity. Abstract Text:

Shahid S Siddiqui,Zeba K Siddiqui,Shahab Uddin,Richard D Minshall,Asrar B Malik,

We show in rat lung microvessel endothelial cells (RLMVEC) that endocytosis is a critical determinant of activation of mitogen-activated protein kinase (MAPK) and thereby regulates endothelial monolayer integrity. In RLMVEC grown in serum-free medium, we observed that albumin supplementation induced the phosphorylation of p38 MAPK within 30 min, which persisted for up to 2 h. Engagement of the endocytic machinery regulated the activation of p38 MAPK that contributed to endothelial cell proliferation and reduction of apoptosis. We also observed an interaction between the caveolar protein caveolin-1 and p38 MAPK with reciprocal coimmunoprecipitation assays and colocalization using double-label immunofluorescence staining. Knockdown of caveolin-1 expression with small interfering RNA significantly reduced endocytosis and activation of p38 MAPK and interfered with the ability of endothelial cells to form a confluent monolayer. Thus caveolae-mediated endocytosis and concomitant activation of p38 MAPK may help to maintain endothelial monolayer integrity by signaling proliferation and survival of endothelial cells.

p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity. Publishing Authors By Initials

SS Siddiqui,ZK Siddiqui,S Uddin,RD Minshall,AB Malik,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: mitogen-activated protein kinases: p38 mitogen-activated protein kinases research

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p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Lung cellular and

VOLUME: 292

Page Numbers: L114-24

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 4

MONTH: 08

YEAR: 2006

p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901229

p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity. Keywords Mesh Terms:

KEYWORDS: p38 Mitogen-Activated Protein Kinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity. Information

Substance Name: p38 Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for p38 MAPK activation coupled to endocytosis is a determinant of endothelial monolayer integrity.

AFFILIATION: Department of Pharmacology, University of Illinois College of Medicine, 835 South Wolcott Ave. (M/C 868), Chicago, IL 60612, USA. ssiddiqu@uic.edu

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL 71626

ACRONYM: HL

MEDLINETA: Am J Physiol Lung Cell Mol Phy

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