p300 expression repression by hypermethylation associated with tumour invasion and metastasis in oesophageal squamous cell carcinoma.

p300 expression repression by hypermethylation associated with tumour invasion and metastasis in oesophageal squamous cell carcinoma. Research Abstract Details 

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p300 expression repression by hypermethylation associated with tumour invasion and metastasis in oesophageal squamous cell carcinoma. Abstract Text:

Changsong Zhang,Ke Li,Lixin Wei,Zhengyou Li,Ping Yu,Lijuan Teng,Kusheng Wu,Jin Zhu,Changsong Zhang,Ke Li,Lixin Wei,Zhengyou Li,Ping Yu,Lijuan Teng,Kusheng Wu,Jin Zhu,

BACKGROUND: Aberrant promoter methylation is an important mechanism for gene silencing. AIMS: To evaluate the promoter methylation status of p300 gene in patients with oesophageal squamous cell carcinoma (OSCC). METHODS: The methylation status of p300 promoter was analysed by methylation-specific PCR (MSP) in 50 OSCC tissues and the matching non-cancerous tissues. Oesophageal cancer cell lines (ECa-109 and TE-10) were treated with the demethylation agent 5-aza-2'-deoxycytidine (5-Aza-CdR), and p300 mRNA expression was detected by RT-PCR. RESULTS: p300 methylation was found in 42% (21/50) of the OSCC tissues, but in only 20% (10/50) of the corresponding non-cancerous tissues (p = 0.017). In OSCC samples, 65% of those with deep tumour invasion (adventitia) and 63% samples with metastasis revealed p300 promoter methylation (p<0.05). p300 mRNA expression was observed in 19.0% (4/21) of methylated tumours and 58.6% (17/29) of unmethylated tumours (p = 0.005). In addition, p300 mRNA expression was observed in 40% (4/10) of methylated non-neoplastic tissues and 87.5% (35/40) of unmethylated non-tumours (p = 0.001). The demethylation caused by 5-Aza-CdR increased the p300 mRNA expression levels in oesophageal cancer cell lines. CONCLUSIONS: p300 transcription silenced by promoter hypermethylation could play a role in the pathogenesis of oesophageal squamous cell carcinoma.

p300 expression repression by hypermethylation associated with tumour invasion and metastasis in oesophageal squamous cell carcinoma. Publishing Authors By Initials

C Zhang,K Li,L Wei,Z Li,P Yu,L Teng,K Wu,J Zhu,C Zhang,K Li,L Wei,Z Li,P Yu,L Teng,K Wu,J Zhu,

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p300 expression repression by hypermethylation associated with tumour invasion and metastasis in oesophageal squamous cell carcinoma. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of clinical pathology

VOLUME: 60

Page Numbers: 1249-53

Journal Abbreviation: J. Clin. Pathol.

ISSN: 1472-4146

DAY: 29

MONTH: Nov

YEAR: 2007

p300 expression repression by hypermethylation associated with tumour invasion and metastasis in oesophageal squamous cell carcinoma. Information

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LANGUAGE: eng

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Grant and Affiliation Information for p300 expression repression by hypermethylation associated with tumour invasion and metastasis in oesophageal squamous cell carcinoma.

AFFILIATION: Department of Cancer Molecular Epidemiology, Shantou University Medical College, Shantou, Guangdong, China.

Country: England

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MEDLINETA: J Clin Pathol

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