p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats.

p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats. Abstract Text:

Pouneh Nouri,Pritmohinder Gill,Min Li,Christopher S Wilcox,William J Welch,

Angiotensin II (ANG II) infusion increases renal superoxide (O(2)(-)) and enhances renal vasoconstriction via macula densa (MD) regulation of tubuloglomerular feedback, but the mechanism is unclear. We targeted the p22(phox) subunit of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) with small-interfering RNA (siRNA) to reduce NADPH oxidase activity and blood pressure response to ANG II in rats. We compared single nephron glomerular filtration rate (SNGFR) in samples collected from the proximal tubule (PT), which interrupts delivery to the MD, and from the distal tubule (DT), which maintains delivery to the MD, to assess MD regulation of GFR. SNGFR was measured in control and ANG II-infused rats (200 ng.kg(-1).min(-1) for 7 days) 2 days after intravenous injection of vehicle or siRNA directed to p22(phox) to test the hypothesis that p22(phox) mediates MD regulation of SNGFR during ANG II. The regulation of SNGFR by MD, determined by PT SNGFR-DT SNGFR, was not altered by siRNA in control rats (control + vehicle, 13 +/- 1, n = 8; control + siRNA, 12 +/- 2 nl/min, n = 8; not significant) but was reduced by siRNA in ANG II-treated rats (ANG II + vehicle, 13 +/- 2, n = 7; ANG II + siRNA, 7 +/- 1 nl/min, n = 8; P < 0.05). We conclude that p22(phox) and NADPH oxidase regulate the SNGFR during ANG II infusion via MD-dependent mechanisms.

p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats. Publishing Authors By Initials

P Nouri,P Gill,M Li,CS Wilcox,WJ Welch,

For similar chemical actions and uses: pharmacologic actions: therapeutic uses: cardiovascular agents: vasoconstrictor agents research abstracts see: chemical actions and uses: pharmacologic actions: therapeutic uses: cardiovascular agents: vasoconstrictor agents research

PUBMED ID PMID:

MEDLINE DATE:

p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Heart and circulat

VOLUME: 292

Page Numbers: H1685-9

Journal Abbreviation:

ISSN: 0363-6135

DAY: 12

MONTH: 01

YEAR: 2007

p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901228

p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats. Keywords Mesh Terms:

KEYWORDS: Vasoconstrictor Agents

MESH TERMS: pharmacology

Chemical & Substance for Abstract: p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats. Information

Substance Name: p22-phox protein, rat

Registry Number: EC 1.6.3.1

Grant and Affiliation Information for p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats.

AFFILIATION: Department of Medicine, Georgetown University, Washington, District of Columbia 20057, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: P01-HL-68686-06

ACRONYM: HL

MEDLINETA: Am J Physiol Heart Circ Physio

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats Related Publications

• 18F-fluoroacetate: a potential acetate analog for prostate tumor imaging--in vivo evaluation of 18F-fluoroacetate versus 11C-acetate.
• Advances in the production, processing and microPET image quality of technetium-94m.
• Asbestos exposure causes mesothelioma, but not this asbestos exposure: an amicus brief to the Michigan Supreme Court.
• Bacterial postgenomics: the promise and peril of systems biology.
• Bioinformatics: the role and limitations of patents.
• Corneal minimal visible lesion thresholds for 2.0 microm laser radiation.
• Esophagogastric cancers secondary to a breast primary tumor do not require resection.
• Hn05 an analysis of complications following surgical treatment of benign parotid disease.
• Improving sensitivity in chiral supercritical fluid chromatography for analysis of active pharmaceutical ingredients.
• In vivo gene expression analysis identifies genes required for enhanced colonization of the mouse urinary tract by uropathogenic Escherichia coli strain CFT073 dsdA.
• Inhibition of isolated Mycobacterium tuberculosis fatty acid synthase I by pyrazinamide analogs.
• Large calcifications in ovaries otherwise normal on ultrasound.
• Letter from the editor.
• Low dose combination of morphine and delta9-tetrahydrocannabinol circumvents antinociceptive tolerance and apparent desensitization of receptors.
• Membrane translocation of P-Rex1 is mediated by G protein betagamma subunits and phosphoinositide 3-kinase.
• Metastasis suppressors and their roles in breast carcinoma.
• Modular determinants of antimicrobial activity in platelet factor-4 family kinocidins.
• NSA and the Japan Stroke Society.
• Osteopontin knockdown suppresses tumorigenicity of human metastatic breast carcinoma, MDA-MB-435.
• RNAi specificity: how big of an issue is it?
• Small-animal PET of tumor angiogenesis using a (76)Br-labeled human recombinant antibody fragment to the ED-B domain of fibronectin.
• Strategic use of preparative chiral chromatography for the synthesis of a preclinical pharmaceutical candidate.
• Sumatriptan and migraine-induced stroke: The news is not all bad!
• Surgical treatment of chronic parotid sialadenitis.
• Switchable reflector in the Panamanian tortoise beetle Charidotella egregia (Chrysomelidae: Cassidinae).
• Synergy between delta9-tetrahydrocannabinol and morphine in the arthritic rat.
• The Escherichia coli argW-dsdCXA genetic island is highly variable, and E. coli K1 strains commonly possess two copies of dsdCXA.
• The antinociceptive effect of Delta9-tetrahydrocannabinol in the arthritic rat involves the CB(2) cannabinoid receptor.
• The drug facts box: providing consumers with simple tabular data on drug benefit and harm.
• p22phox in the macula densa regulates single nephron GFR during angiotensin II infusion in rats.