Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production.

Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production. Research Abstract Details 

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Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production. Abstract Text:

Shawn D Harmon,Xiang Fang,Terry L Kaduce,Shanming Hu,V Raj Gopal,John R Falck,Arthur A Spector,

Cytochrome P450 (CYP) omega-oxidases convert arachidonic acid (AA) to 20-hydroxyeicosatetraenoic acid (20-HETE), a lipid mediator that modulates vascular tone. We observed that a microsomal preparation containing recombinant human CYP4F3B, which converts AA to 20-HETE, converted eicosapentaenoic acid (EPA) to 20-OH-EPA. Likewise, docosahexaenoic acid (DHA) was converted to 22-OH-DHA, indicating that human CYP4F3B also can oxidize 22-carbon omega-3 fatty acids. Consistent with these findings, addition of 0.5-5 microM EPA, DHA or omega-3 docosapentaenoic acid (DPA) to incubations containing 0.5 microM [3H]AA inhibited [3H]20-HETE production by 15-65%. [3H]20-OH-EPA was rapidly taken up by COS-7 cells, and almost all of the incorporated radioactivity remained as unmodified 20-OH-EPA. The 20-OH-EPA stimulated luciferase activity in COS-7 cells that express peroxisome proliferator-activated receptor alpha, indicating that this EPA metabolite may function as a lipid mediator. These findings suggest that some functional effects of omega-3 fatty acid supplementation may be due to inhibition of 20-HETE formation or the conversion of EPA to the corresponding omega-oxidized product.

Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production. Publishing Authors By Initials

SD Harmon,X Fang,TL Kaduce,S Hu,V Raj Gopal,JR Falck,AA Spector,

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Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Prostaglandins, leukotrienes, and essential fatty

VOLUME: 75

Page Numbers: 169-77

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ISSN: 0952-3278

DAY: 3

MONTH: 07

YEAR: 2006

Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production. Information

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LANGUAGE: eng

NlmUniqueID: 8802730

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AFFILIATION: Department of Biochemistry, Carver College of Medicine, 4-403 BSB, University of Iowa, Iowa City, IA 52242, USA.

Country: Scotland

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MEDLINETA: Prostaglandins Leukot Essent F

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