Oxygen metabolism by neuronal nitric-oxide synthase.

Oxygen metabolism by neuronal nitric-oxide synthase. Research Abstract Details 

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Oxygen metabolism by neuronal nitric-oxide synthase. Abstract Text:

Ying Tong Gao,Satya Prakash Panda,Linda J Roman,Pavel ,Yuzuru Ishimura,Bettie Sue S Masters,

Nitric-oxide synthases (NOS) catalyze nitric oxide (NO) formation from the amino acid L-arginine. NOS is known to catalyze more than one reaction: the NO-producing reaction is considered to be the coupled reaction, and the uncoupled reactions are those that produce reactive (reduced) oxygen species (ROS), such as superoxide anion (O-2.) and/or hydrogen peroxide (H2O2). As an oxygenase, NOS has been known for more than two decades, yet there is no complete description of oxygen stoichiometry. The present paper is focused on oxygen stoichiometry and the effects of cofactor binding on the neuronal isoform (nNOS) on oxygen uptake and product formation. Products of the uncoupled reactions are analyzed using diacetyldeuteroheme-substituted horseradish peroxidase as a trapping agent for both O-2. and H2O2. The addition of calmodulin not only stimulated the oxygen uptake rate but also changed the product of the uncoupled reaction, supporting the possibility of two different sites for electron leakage to molecular oxygen. Quantitative analysis of the uncoupled (substrate-free) reaction revealed a stoichiometry close to the theoretical value, and adding L-arginine not only initiates the coupled reaction, but also inhibits oxygen uptake. The presence of tetrahydrobiopterin affects oxygen metabolism by lowering the apparent Km value of nNOS for oxygen in the uncoupled reaction.

Oxygen metabolism by neuronal nitric-oxide synthase. Publishing Authors By Initials

YT Gao,SP Panda,LJ Roman,P ,Y Ishimura,BS Masters,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Oxygen metabolism by neuronal nitric-oxide synthase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 7921-9

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 17

MONTH: 01

YEAR: 2007

Oxygen metabolism by neuronal nitric-oxide synthase. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

Oxygen metabolism by neuronal nitric-oxide synthase. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: chemistry

Chemical & Substance for Abstract: Oxygen metabolism by neuronal nitric-oxide synthase. Information

Substance Name: Nitric Oxide Synthase Type I

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Oxygen metabolism by neuronal nitric-oxide synthase.

AFFILIATION: Department of Biochemistry, University of Texas Health Science Center, San Antonio, Texas 78229-3900, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL30050

ACRONYM: HL

MEDLINETA: J Biol Chem

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