Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding.

Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. Research Abstract Details 

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Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. Abstract Text:

Sydney M Evans,Amy E Schrlau,Ara A Chalian,Paul Zhang,Cameron J Koch,

The oxygen status of skin is a controversial topic. Skin is radiosensitive, suggesting it is well-oxygenated. However, it can be further sensitized with nitroimidazole drugs, implying that it is partially hypoxic. Skin oxygen levels are difficult to measure with either electrodes or the hypoxia-monitoring agent (3)H-misonidazole. For the latter, binding has previously been reported to be high in murine skin, but this could be attributed to either non-oxygen-dependent variations in nitroreductase activity, drug metabolism, and/or actual oxygen gradients. We obtained tumor and skin from patients given EF5, a 2-nitroimidazole tissue hypoxia monitor. We performed immunohistochemical studies using highly specific monoclonal antibodies for the hypoxia-dependent production of EF5 tissue adducts. Some tissue sections were counterstained using either Ki67 for proliferation or CD31 for vessels. We found that the human dermis is well-oxygenated, the epidermis is modestly hypoxic and portions of some sebaceous glands and hair follicles are moderately to severely hypoxic. Normal and irradiated skin had similar oxygenation patterns. Control studies demonstrated that these observations are not due to tissue variations in nitroreductase activity. The importance of the highly heterogeneous distribution of oxygen in skin requires further study, but recent investigations suggest that skin hypoxia may have important clinical ramifications including mediating cellular transformation.

Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. Publishing Authors By Initials

SM Evans,AE Schrlau,AA Chalian,P Zhang,CJ Koch,

For similar biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research

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Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of investigative dermatology

VOLUME: 126

Page Numbers: 2596-606

Journal Abbreviation: J. Invest. Dermatol.

ISSN: 1523-1747

DAY: 29

MONTH: 06

YEAR: 2006

Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. Information

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LANGUAGE: eng

NlmUniqueID: 426720

Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. Keywords Mesh Terms:

KEYWORDS: Tissue Distribution

MESH TERMS: radiation effects

Chemical & Substance for Abstract: Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding. Information

Substance Name: Nitroreductases

Registry Number: EC 1.7.-

Grant and Affiliation Information for Oxygen levels in normal and previously irradiated human skin as assessed by EF5 binding.

AFFILIATION: Department of Radiation Oncology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. sydevans@mail.med.upenn.edu

Country: United States

AGENCY: United States NCI

GRANT: R01 CA75285

ACRONYM: CA

MEDLINETA: J Invest Dermatol

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