Oxygen-dependent differentiation of human keratinocytes.

Oxygen-dependent differentiation of human keratinocytes. Research Abstract Details 

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Oxygen-dependent differentiation of human keratinocytes. Abstract Text:

Mai A Ngo,Nadezda N Sinitsyna,Qin Qin,Robert H Rice,

Oxygen is an essential micronutrient. Unlike many internal tissues, human epidermis obtains much of its oxygen supply from the atmosphere (21% oxygen), and it ordinarily experiences higher oxygen levels than internal tissues (estimated approximately 5%). To test whether epidermal cell growth and differentiation depend upon this higher oxygen level, keratinocyte cultures were studied at 21, 5, and 2% oxygen concentrations. Compared to 21% oxygen, culture in 5% had little effect on growth but led to profound suppression of the differentiation program as assessed by expression of differentiation markers and formation of squames in the superficial layers. Culture in 2% oxygen reduced the growth rate as well as stratification and differentiation. In low-oxygen conditions, the cells exhibited increased colony-forming ability, consistent with a lower proportion of differentiated cells, and increased expression of vascular endothelial growth factor and cyclooxygenase-2. Growth in 21% oxygen led to higher levels of glutathione and expression of oxidant-responsive genes. Electrophoretic mobility supershift assay using an involucrin activator protein 1 (AP1) response element sequence revealed altered binding by proteins of the Jun and Fos families in nuclear extracts. The present data thus demonstrate oxygen-dependent differentiation in human keratinocytes, to which altered utilization of AP1 transcriptional response elements may contribute.

Oxygen-dependent differentiation of human keratinocytes. Publishing Authors By Initials

MA Ngo,NN Sinitsyna,Q Qin,RH Rice,

For similar peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research abstracts see: peptides: intercellular signaling peptides and proteins: angiogenic proteins: vascular endothelial growth factors: vascular endothelial growth factor a research

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Oxygen-dependent differentiation of human keratinocytes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of investigative dermatology

VOLUME: 127

Page Numbers: 354-61

Journal Abbreviation: J. Invest. Dermatol.

ISSN: 1523-1747

DAY: 14

MONTH: 09

YEAR: 2006

Oxygen-dependent differentiation of human keratinocytes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 426720

Oxygen-dependent differentiation of human keratinocytes. Keywords Mesh Terms:

KEYWORDS: Vascular Endothelial Growth Factor A

MESH TERMS: metabolism

Chemical & Substance for Abstract: Oxygen-dependent differentiation of human keratinocytes. Information

Substance Name: Cyclooxygenase 2

Registry Number: EC 1.14.99.1

Grant and Affiliation Information for Oxygen-dependent differentiation of human keratinocytes.

AFFILIATION: Department of Environmental Toxicology, University of California, Davis, California 95616-8588, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: R01 AR27130

ACRONYM: AR

MEDLINETA: J Invest Dermatol

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