Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo.

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo. Abstract Text:

Mark T Johnson,Edward A Freeman,David K Gardner,Patricia A Hunt,

The requirement for oxidative metabolism of pyruvate during oogenesis in vivo was evaluated by inactivating Pdha1, a gene encoding an enzymatic subunit of pyruvate dehydrogenase complex, in murine oocytes at the beginning of the follicular growth phase. Immunohistochemical analysis revealed that Pdha1(-) oocytes have dramatically reduced amounts of pyruvate dehydrogenase enzyme by the secondary follicle stage. Despite this reduction, these oocytes grow to normal size, are ovulated, and can be fertilized. Pdha1(-) oocytes are, however, impaired in their ability to support embryonic development, as demonstrated by the failure of fertilized oocytes to develop beyond the one-cell zygote stage in vivo. Immunocytochemical evaluation showed that almost all (98.4%) ovulated Pdha1(-) oocytes have not completed meiotic maturation and/or have gross abnormalities of the meiotic spindle and chromatin. Meiotic maturation is even more compromised when these oocytes are matured in vitro in the absence of cumulus cells or in the presence of the gap junction inhibitor 18-alpha glycyrrhetinic acid, indicating that cumulus cells can partially compensate for this enzymatic deficiency through a gap junction-mediated mechanism. Ovulated Pdha1(-) oocytes were also shown to have reduced levels of total ATP content and NAD(P)H autofluorescence relative to oocytes without this enzymatic deficiency. These studies demonstrate that oxidative metabolism of pyruvate is essential for proper completion of oogenesis, serving as a vital source of energy during meiotic maturation. At earlier stages of oogenesis this metabolic pathway may not be necessary due to metabolic compensation by the granulosa cells.

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo. Publishing Authors By Initials

MT Johnson,EA Freeman,DK Gardner,PA Hunt,

For similar organic chemicals: carboxylic acids: keto acids: pyruvates: pyruvic acid research abstracts see: organic chemicals: carboxylic acids: keto acids: pyruvates: pyruvic acid research

PUBMED ID PMID:

MEDLINE DATE:

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biology of reproduction

VOLUME: 77

Page Numbers: 2-8

Journal Abbreviation: Biol. Reprod.

ISSN: 0006-3363

DAY: 21

MONTH: 02

YEAR: 2007

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 207224

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo. Keywords Mesh Terms:

KEYWORDS: Pyruvic Acid

MESH TERMS: metabolism

Chemical & Substance for Abstract: Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo. Information

Substance Name: pyruvate dehydrogenase E1alpha subunit

Registry Number: EC 1.2.4.1

Grant and Affiliation Information for Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo.

AFFILIATION: Department of Genetics, Case School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA. mtj@case.edu

Country: United States

AGENCY: United States NIEHS

GRANT: R01 ES013527

ACRONYM: ES

MEDLINETA: Biol Reprod

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo Related Publications

• Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.
• Analysis of the cellular infiltration of benzyl-esterified hyaluronan sponges implanted in rats.
• Another cause of 'pseudophaeochromocytoma'--quetiapine associated with a false positive normetanephrine result.
• Anti-apoptotic peptides protect against radiation-induced cell death.
• Buffering airway acid decreases exhaled nitric oxide in asthma.
• Characterization of histones and their post-translational modifications by mass spectrometry.
• Chemical derivatization of histones for facilitated analysis by mass spectrometry.
• Differential cellular expression of LIGHT and its receptors in early gestation human placentas.
• Differential expression of human leukocyte antigen-G (HLA-G) messenger RNAs and proteins in normal human prostate and prostatic adenocarcinoma.
• Effect of vascular cannulation on serum concentrations of LH, FSH, prolactin and cortisol in prepubertal gilts.
• Electromechanical carbon nanotube switches for high-frequency applications.
• Flight and fight: a comparative view of the neurophysiology and genetics of honey bee defensive behavior.
• Hn04 genetics of head and neck paraganglioma.
• Lactate stimulates endothelial cell migration.
• Lightning, sudden cardiac death, simulation and an automated external defibrillator: the perfect storm.
• Microtubule assembly dynamics at the nanoscale.
• Monitoring the neonatal brain: a survey of current practice among Australian and New Zealand neonatologists.
• Myocardial infarction in young patients without coronary atherosclerosis: assume primary antiphospholipid syndrome until proved otherwise.
• Organismal differences in post-translational modifications in histones H3 and H4.
• Ovarian follicular response of mares to GnRH agonist (leuprolide acetate) treatment after pituitary suppression.
• Oxidative metabolism of pyruvate is required for meiotic maturation of murine oocytes in vivo.
• Protein profile of osteoarthritic human articular cartilage using tandem mass spectrometry.
• Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.
• Reproducibility of impaired glucose tolerance (IGT) and impaired fasting glucose (IFG) classification: a systematic review.
• Schizophrenia in childhood.
• Stranger in a strange land.
• The relationship between body mass index and per cent body fat in the severely obese.
• The source and distribution of polychlorinated dibenzo-p-dioxin and polychlorinated dibenzofurans in sediments of Port Jackson, Australia.
• Vitreous modulation of gene expression in low-passage human retinal pigment epithelial cells.
• Within-state availability of transition-to-adulthood services for youths with serious mental health conditions.