Oxidation of Prx2 and phosphorylation of GRP58 by angiotensin II in human coronary smooth muscle cells identified by 2D-DIGE analysis.

Oxidation of Prx2 and phosphorylation of GRP58 by angiotensin II in human coronary smooth muscle cells identified by 2D-DIGE analysis. Research Abstract Details 

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Oxidation of Prx2 and phosphorylation of GRP58 by angiotensin II in human coronary smooth muscle cells identified by 2D-DIGE analysis. Abstract Text:

Yoshiko Tokutomi,Norie Araki,Keiichiro Kataoka,Eiichiro Yamamoto,Shokei Kim-Mitsuyama,Yoshiko Tokutomi,Norie Araki,Keiichiro Kataoka,Eiichiro Yamamoto,Shokei Kim-Mitsuyama,

To study early changes in angiotensin II (Ang II)-induced signaling with post-translational modifications, we analyzed proteins from cultured human coronary smooth muscle cells stimulated with Ang II, using two-dimensional difference gel electrophoresis (2D-DIGE) combined with ProQ Diamond and SYPRO Ruby staining, followed by mass spectrometry or Western blotting. Among 40 proteins identified, peroxiredoxin 2 (Prx2) was oxidized and 58 kDa glucose-regulated protein (GRP58) was phosphorylated after 5 min of Ang II (1 microM) stimulation. Valsartan, a selective Ang II type 1 (AT1) receptor blocker, and N-acetylcysteine, an antioxidant, inhibited both of these modifications, indicating the contribution of AT1 receptor and reactive oxygen species to oxidation of Prx2 and phosphorylation of GRP58 by Ang II.

Oxidation of Prx2 and phosphorylation of GRP58 by angiotensin II in human coronary smooth muscle cells identified by 2D-DIGE analysis. Publishing Authors By Initials

Y Tokutomi,N Araki,K Kataoka,E Yamamoto,S Kim-Mitsuyama,Y Tokutomi,N Araki,K Kataoka,E Yamamoto,S Kim-Mitsuyama,

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Oxidation of Prx2 and phosphorylation of GRP58 by angiotensin II in human coronary smooth muscle cells identified by 2D-DIGE analysis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemical and biophysical research communication

VOLUME: 364

Page Numbers: 822-30

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 25

MONTH: 10

YEAR: 2007

Oxidation of Prx2 and phosphorylation of GRP58 by angiotensin II in human coronary smooth muscle cells identified by 2D-DIGE analysis. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Oxidation of Prx2 and phosphorylation of GRP58 by angiotensin II in human coronary smooth muscle cells identified by 2D-DIGE analysis.

AFFILIATION: Department of Pharmacology and Molecular Therapeutics, Kumamoto University Graduate School of Medical Sciences, Honjo 1-1-1, Kumamoto 860-8556, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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