Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation.

Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Abstract Text:

Michael W Epperly,Laura D Epperly,YunYun Niu,Hong Wang,Xichen Zhang,Darcy Franicola,Joel S Greenberger,Michael W Epperly,Laura D Epperly,YunYun Niu,Hong Wang,Xichen Zhang,Darcy Franicola,Joel S Greenberger,

We determined whether manganese superoxide dismutase (MnSOD)-plasmid liposome (PL) transfection of C57BL/ 6NHsd mouse bone marrow protected cells irradiated at room temperature (24 degrees C) or in the cryopreserved state. MnSOD-overexpressing hematopoietic progenitor 2C6 cells were radioresistant compared to the parent 32D cl 3 cells when irradiated frozen or at 24 degrees C. Fresh whole marrow from mice injected intravenously with MnSOD-PL prior to explant as well as explanted marrow single cell suspensions transfected in vitro were irradiated at 24 degrees C or -80 degrees C. In vivo or in vitro transfection of marrow with MnSOD-PL produced significant radiation protection of irradiated marrow progenitor cells compared to controls at 24 degrees C or -80 degrees C. (in vivo transfection D(0) 2.19 +/- 0.21 at 24 degrees C, D(0) 2.10 +/- 0.07 at -80 degrees C compared to control D(0) 1.56 +/- 0.06 or 1.66 +/- 0.04, P = 0.047 and 0.017 respectively; in vitro transfection D(0) 2.35 +/- 0.11 at 24 degrees C, D(0) 3.42 +/- 0.13 at -80 degrees C compared to D(0) 1.81 +/- 0.01 or 2.53 +/- 0.05, P = 0.0087 and 0.0026, respectively). Thus the MnSOD transgene product protects frozen marrow cells as well as marrow cells irradiated at 24 degrees C.

Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Publishing Authors By Initials

MW Epperly,LD Epperly,Y Niu,H Wang,X Zhang,D Franicola,JS Greenberger,MW Epperly,LD Epperly,Y Niu,H Wang,X Zhang,D Franicola,JS Greenberger,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Radiation research

VOLUME: 168

Page Numbers: 560-6

Journal Abbreviation: Radiat. Res.

ISSN: 0033-7587

DAY: 1

MONTH: Nov

YEAR: 2007

Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 401245

Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation.

AFFILIATION: Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA. epperly@pitt.edu

Country: United States

AGENCY: United States PHS

GRANT: U19-A1068021

ACRONYM:

MEDLINETA: Radiat Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation Related Publications

• 7. Immunologic lung disease.
• A novel 5-[1,3,4-oxadiazol-2-yl]-N-aryl-4,6-pyrimidine diamine having dual EGFR/HER2 kinase activity: design, synthesis, and biological activity.
• Aspergillosis--clinical aspects.
• Cellular and in Vivo Activity of JNJ-28871063, A Nonquinazoline Pan-ErbB Kinase Inhibitor That Crosses the Blood-Brain Barrier and Displays Efficacy against Intracranial Tumors.
• Design, synthesis, and evaluation of 3,4-disubstituted pyrazole analogues as anti-tumor CDK inhibitors.
• Discovery of novel 4-amino-6-arylaminopyrimidine-5-carbaldehyde oximes as dual inhibitors of EGFR and ErbB-2 protein tyrosine kinases.
• Effects of MnSOD-plasmid liposome gene therapy on antioxidant levels in irradiated murine oral cavity orthotopic tumors.
• Ethyl pyruvate, a potentially effective mitigator of damage after total-body irradiation.
• Gene therapy approaches for stem cell protection.
• Increased longevity of hematopoiesis in continuous bone marrow cultures derived from NOS1 (nNOS, mtNOS) homozygous recombinant negative mice correlates with radioresistance of hematopoietic and marrow stromal cells.
• Intraesophageal MnSOD-plasmid liposome enhances engraftment and self-renewal of bone marrow derived progenitors of esophageal squamous epithelium.
• Overexpression of the MnSOD transgene product protects cryopreserved bone marrow hematopoietic progenitor cells from ionizing radiation.
• Radioprotection in vitro and in vivo by minicircle plasmid carrying the human manganese superoxide dismutase transgene.
• Reduced irradiation pulmonary fibrosis and stromal cell migration in Smad3-/- marrow chimeric mice.