Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo.

Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo. Abstract Text:

Hua Chen,Qiang Huang,Jun Dong,De-Zhong Zhai,Ai-Dong Wang,Qing Lan,

BACKGROUND: Gliomas are the most common and aggressive primary brain tumors for which unfortunately no effective treatment modalities exist despite advances in molecular biology as the knowledge base to unravel the extremely complex molecular mechanisms of tumorigenesis is limited. In this study an attempt has been made to understand the molecular pathological basis of tumorigenesis which led to an identification of an oncogene, CDC2, and an epigenetic strategy has been evaluated to control the tumorigensis by downregulating this oncogene. METHODS: Tissue microarrays were utilized to investigate the expression of genes in a large number of tumor samples and to identify overexpressed genes which could be potentially causing tumorigenesis. Retroviral vectors expressing short hairpin RNAs (shRNAs) targeted against CDC2 were designed and transducted into human glioma cell line ex vivo in order to downregulate the expression of CDC2. Real-Time PCR was used to determine the level of CDC2 mRNA. Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis. Following ex vivo study, viral particles containing small interfering RNA for CDC2 were subsequently injected into xenogeneic graft tumor of nude mice and the weight of human glioma xenografts, survival and resulting phenotypic changes of target gene were investigated. RESULTS: Human glioma tissue microarrays indicated the positive expression rates of CDC2/CyclinB1 with a positive correlation with pathologic grades (r = 0.982, r = 0.959, respectively). Retroviral vectors expressing short hairpin RNAs (shRNAs) against CDC2 caused efficient deletion of CDC2, cellular G2/M arrest concluding in apoptosis and inhibition of proliferation in human glioma cells U251 and SHG-44 cell lines ex vivo. And the viral particles containing small interfering RNA for CDC2 were subsequently injected into subcutaneous and intracranial xenogeneic graft tuomrs of nude mice. For subcutaneous tumors, injection of CDC2-shRNA retroviruses significantly decreased tumor weight and volume compared with control. Immunohistochemistry indicated that CDC2 are negative and TUNEL are positive in tumors treated with recombinant retrovirus. For mice implanted with intracranial gliomas, treatment of CDC2-shRNA retroviruses increased survival times compared with control. CONCLUSION: CDC2 gene plays an important role in the proliferation of human gliomas. Downregulation of CDC2 could potentialy inhibit human gliomas cells growth ex vivo and in vivo. From these results, it was suggested that CDC2 might be a potential target on gene therapy of human gliomas.

Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo. Publishing Authors By Initials

H Chen,Q Huang,J Dong,DZ Zhai,AD Wang,Q Lan,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC cancer

VOLUME: 8

Page Numbers: 29

Journal Abbreviation: BMC Cancer

ISSN: 1471-2407

DAY: 29

MONTH: 01

YEAR: 2008

Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100967800

Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo.

AFFILIATION: Department of Neurosurgery and Brain Tumor Research Laboratory, 2nd Affiliated Hospital, Soochow University, Suzhou, China. cutv1220@yahoo.com.cn

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: BMC Cancer

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Overexpression of CDC2/CyclinB1 in gliomas, and CDC2 depletion inhibits proliferation of human glioma cells in vitro and in vivo Related Publications

• Anxiety and stress reduction in medical education: an intervention.
• Identification of two sterol carrier protein-2 like genes in the yellow fever mosquito, Aedes aegypti.
• Macrophage migration inhibitory factor induces MMP-9 expression in macrophages via the MEK-ERK MAP kinase pathway.
• Neurogenin 3 recruits CBP co-activator to facilitate histone H3/H4 acetylation in the target gene INSM1.
• Orphan nuclear receptor function in the ovary.
• Pdx-1 modulates histone H4 acetylation and insulin gene expression in terminally differentiated alpha-TC-1 cells.
• Relationship between burnout and occupational stress among nurses in China.
• The Biotransformation of 6-Hydroxyhyoscyamine to Scopolamine by Suspension and Immobilized Cells of Anisodus tanguticus.
• Use of ammoniacal nitrogen tolerant microalgae in landfill leachate treatment.
• [Anatomic study of the supra-infratentorial presigmoid partial labyrinthectomy keyhole approach assisted by neuro-navigation]
• [Anatomic study on the design of far-lateral transcondylar transtubercular keyhole approach assisted by neuro-navigation]
• [Avoiding the interference of ABCD1 pseudogenes in the molecular diagnosis of X-linked adrenoleukodystrophy by double amplification refractory mutation system]
• [Cancer initiating cell theory: popularity and controversies]
• [Clinicopathological characteristics and prognosis of patients with recurrent colorectal cancer]
• [Determination of alpha-methoxynaphthalene by reversed-phase high performance liquid chromatographic method]
• [Expression of cyclin-dependent kinase CDC2 and its significance in malignant progression of gliomas]
• [Increasing activity of the intestinal mucosal mast cells in rats with multiple organ failure]
• [Isolation and culture of tumor stem cells from human brain glioma tissues]
• [Linear scanning analysis of prehistoric human bones in Xigongqiao Site, Tengzhou, Shandong Province by use of SEM-EDS]
• [Linguistic-cultural differences between Chinese and Western medicine and English translation of Chinese medicine]
• [Multiple auditory steady-state responses to bone conduction stimuli]
• [Norm study of occupational stress on the professionals in Chengdu and Chongqing area]
• [Rapid diagnosis of 21 trisomy syndrome by fluorescence quantitative polymerase chain reaction]
• [Relationship between interleukin-6 gene polymorphism and coronary heart disease and its effect on plasma lipid levels]
• [Screen and identification of Streptocococcus pneumoniae genes specifically induced in host]
• [Study of the occupational stress norm and it's application for the executive group and administrative support group]
• [Surgery therapy of Crohn disease complicated with fistula]
• [The influence of Huang di nei jing (Huangdi's inner classic) on Shuo wen jie zi (the origin of Chinese characters)]
• [The scale and application of the norm of occupational stress on the professionals in Chengdu and Chongqing area]
• [Use of bicyclol in treatment of chronic hepatitis B virus infection: a systematic review]