Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells.

Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Research Abstract Details 

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Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Abstract Text:

Weibiao Huang,George H Rudkin,Brian Carlsen,Kenji Ishida,Peyman Ghasri,Bardia Anvar,Dean T Yamaguchi,Timothy A Miller,

Bone morphogenetic proteins (BMP) play a pivotal role in growth and differentiation of osteoblastic lineage cells. BMPs are potent stimulators of bone formation in various animal models. To understand the mechanism of BMP action in bone cells, we have investigated the effects of overexpression of the BMP-2 gene on proliferation and differentiation of UMR-106 rat osteosarcoma cells. A stable UMR-106 cell line overexpressing the BMP-2 gene was established by transfection of cells using a mammalian expression vector harboring human BMP-2 cDNA followed by G418 selection. After introduction of the BMP-2 gene, UMR-106 cells appeared more spindle-shaped in morphology compared to the predominantly cuboidal appearance of the parental cells. Overexpression of BMP-2 markedly inhibited proliferation as measured by cell counting and [3H]thymidine incorporation assays. Extracellular matrix (ECM) derived from cells overexpressing BMP-2 exhibited a less supportive effect on proliferation of UMR cells than did ECM derived from parental cells. Furthermore, cell-cell communication through gap junctions was reduced more than 50% as determined by nondisruptive fluorescent dye transfer assays. Overexpression of BMP-2 significantly stimulated expression of osteocalcin and alkaline phosphatase genes, indicating its role in osteoblastic differentiation. There was little effect on osteopontin gene expression.

Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Publishing Authors By Initials

W Huang,GH Rudkin,B Carlsen,K Ishida,P Ghasri,B Anvar,DT Yamaguchi,TA Miller,

For similar genetic processes: gene expression regulation: up-regulation research abstracts see: genetic processes: gene expression regulation: up-regulation research

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Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Experimental cell research

VOLUME: 274

Page Numbers: 226-34

Journal Abbreviation: Exp. Cell Res.

ISSN: 0014-4827

DAY: 1

MONTH: Apr

YEAR: 2002

Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Information

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LANGUAGE: eng

NlmUniqueID: 373226

Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Keywords Mesh Terms:

KEYWORDS: Up-Regulation

MESH TERMS: genetics

Chemical & Substance for Abstract: Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells.

AFFILIATION: Plastic Surgery Section, Veterans Administration Greater Los Angeles Healthcare System, Los Angeles, California 90073, USA.

Country: United States

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MEDLINETA: Exp Cell Res

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