Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation.

Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation. Research Abstract Details 

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Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation. Abstract Text:

Jianmin Fang,Felix A Ruiz,Melissa Docampo,Shuhong Luo,Juliany C F Rodrigues,Lucimar S Motta,Peter Rohloff,Roberto Docampo,Jianmin Fang,Felix A Ruiz,Melissa Docampo,Shuhong Luo,Juliany C F Rodrigues,Lucimar S Motta,Peter Rohloff,Roberto Docampo,

We report the cloning, expression, purification, and characterization of the Trypanosoma cruzi exopolyphosphatase (TcPPX). The product of this gene (TcPPX), has 383 amino acids and a molecular mass of 43.1 kDa. TcPPX differs from most exopolyphosphatases in its preference for short-chain polyphosphate (poly P). Heterologous expression of TcPPX in Escherichia coli produced a functional enzyme that had a neutral optimum pH and was dramatically inhibited by low concentrations of Zn2+, high concentrations of basic amino acids (lysine and arginine), and heparin. TcPPX is a processive enzyme and does not hydrolyze ATP, pyrophosphate, or p-nitrophenyl phosphate, although it hydrolyzes guanosine 5'-tetraphosphate very efficiently. Overexpression of TcPPX resulted in a dramatic decrease in total short-chain poly P and partial decrease in long-chain poly P. This was accompanied by a delayed regulatory volume decrease after hyposmotic stress. These results support the role of poly P in T. cruzi osmoregulation.

Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation. Publishing Authors By Initials

J Fang,FA Ruiz,M Docampo,S Luo,JC Rodrigues,LS Motta,P Rohloff,R Docampo,J Fang,FA Ruiz,M Docampo,S Luo,JC Rodrigues,LS Motta,P Rohloff,R Docampo,

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Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 32501-10

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 7

MONTH: 09

YEAR: 2007

Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

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Grant and Affiliation Information for Overexpression of a Zn2+-sensitive soluble exopolyphosphatase from Trypanosoma cruzi depletes polyphosphate and affects osmoregulation.

AFFILIATION: Center for Tropical and Emerging Global Diseases and Department of Cellular Biology, University of Georgia, Paul D. Coverdell Biomedical and Health Sciences Center, Athens, Georgia 30602, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI68647

ACRONYM: AI

MEDLINETA: J Biol Chem

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