Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance.

Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Research Abstract Details 

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Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Abstract Text:

Chan Ju Lim,Jung Eun Hwang,Huan Chen,Joon Ki Hong,Kyung Ae Yang,Man Soo Choi,Kyun Oh Lee,Woo Sik Chung,Sang Yeol Lee,Chae Oh Lim,

The dehydration responsive element binding protein 2 (DREB2) subgroup belongs to the plant-specific APETALA2/ethylene-responsive element binding factor (AP2/ERF) family of transcription factors. We have characterized cDNA encoding Arabidopsis thaliana DREB2C, which is induced by mild heat stress. Both an electrophoretic mobility shift assay (EMSA) and a yeast one-hybrid assay revealed that DREB2C(145-528) was able to form a complex with the dehydration responsive element/C-repeat (DRE/CRT; A/GCCGAC) motif. A trans-activating ability test in yeast demonstrated that DREB2C could effectively function as a trans-activator. Constitutive expression of DREB2C under the control of the cauliflower mosaic virus (CaMV) 35S promoter led to enhanced thermotolerance in transgenic lines of Arabidopsis. Microarray and RT-PCR analyses of transgenic plants revealed that DREB2C regulates expression of several heat stress-inducible genes that contain DRE/CRT elements in their promoters. From these data, we deduced that DREB2C is a regulator of heat stress tolerance in Arabidopsis.

Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Publishing Authors By Initials

CJ Lim,JE Hwang,H Chen,JK Hong,KA Yang,MS Choi,KO Lee,WS Chung,SY Lee,CO Lim,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemical and biophysical research communication

VOLUME: 362

Page Numbers: 431-6

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 0006-291X

DAY: 10

MONTH: 08

YEAR: 2007

Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Information

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LANGUAGE: eng

NlmUniqueID: 372516

Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: genetics

Chemical & Substance for Abstract: Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance. Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance.

AFFILIATION: Division of Applied Life Science (BK21), Environmental Biotechnology National Core Research Center and PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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