Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation.

Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Abstract Text:

David J Kennedy,Sandeep Vetteth,Miaorong Xie,Sankaridrug M Periyasamy,Zijian Xie,Chi Han,Venkatesha Basrur,Krishna Mutgi,Vladimir Fedorov,Deepak Malhotra,Joseph I Shapiro,

The effect of cardiac glycosides to increase cardiac inotropy by altering Ca(2+) cycling is well known but still poorly understood. The studies described in this report focus on defining the effects of ouabain signaling on sarcoplasmic reticulum Ca(2+)-ATPase function. Rat cardiac myocytes treated with 50 microM ouabain demonstrated substantial increases in systolic and diastolic Ca(2+) concentrations. The recovery time constant for the Ca(2+) transient, tau(Ca(2+)), was significantly prolonged by ouabain. Exposure to 10 microM H(2)O(2), which causes an increase in intracellular reactive oxygen species similar to that of 50 microM ouabain, caused a similar increase in tau(Ca(2+)). Concurrent exposure to 10 mM N-acetylcysteine or an aqueous extract from green tea (50 mg/ml) both prevented the increases in tau(Ca(2+)) as well as the changes in systolic or diastolic Ca(2+) concentrations. We also observed that 50 microM ouabain induced increases in developed pressure in addition to diastolic dysfunction in the isolated perfused rat heart. Coadministration of ouabain with N-acetylcysteine prevented these increases. Analysis of sarcoplasmic reticulum Ca(2+)-ATPase protein revealed increases in both the oxidation and nitrotyrosine content in the ouabain-treated hearts. Liquid chromatography-mass spectrometric analysis confirmed that the sarcoplasmic reticulum Ca(2+)-ATPase protein from ouabain-treated hearts had modifications consistent with oxidative and nitrosative stress. These data suggest that ouabain induces oxidative changes of the sarcoplasmic reticulum Ca(2+)-ATPase structure and function that may, in turn, produce some of the associated changes in Ca(2+) cycling and physiological function.

Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Publishing Authors By Initials

DJ Kennedy,S Vetteth,M Xie,SM Periyasamy,Z Xie,C Han,V Basrur,K Mutgi,V Fedorov,D Malhotra,JI Shapiro,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Heart and circulat

VOLUME: 291

Page Numbers: H3003-11

Journal Abbreviation: Am. J. Physiol. Heart Circ. Ph

ISSN: 0363-6135

DAY: 21

MONTH: 07

YEAR: 2006

Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901228

Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Keywords Mesh Terms:

KEYWORDS: Sarcoplasmic Reticulum Calcium-Transport

MESH TERMS: metabolism

Chemical & Substance for Abstract: Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation. Information

Substance Name: Sarcoplasmic Reticulum Calcium-Transport

Registry Number: EC 3.6.3.8

Grant and Affiliation Information for Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation.

AFFILIATION: Dept. of Medicine, Medical University of Ohio, Toledo, Ohio 43614-5089, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL-67963

ACRONYM: HL

MEDLINETA: Am J Physiol Heart Circ Physio

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Ouabain decreases sarcoendoplasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation Related Publications

• 3-month-old boy with a febrile urinary tract infection.
• American academy of pediatrics policy statements on circumcision and urinary tract infection.
• Bilateral integrative medicine, obviously.
• Characterization of ATP-independent ERK inhibitors identified through in silico analysis of the active ERK2 structure.
• Cryptorchidism and overactive bladder.
• Dermatitis in infants and children; diagnosis and management.
• Dynamic contrast-enhanced magnetic resonance imaging for the evaluation of hydronephrosis in children.
• Early clinical experience with monti procedure and managing the nonpalpable testis in children.
• Expectant management in neonatal reflux.
• Incontinence in a child with a duplex kidney: case report.
• Long-term outcomes of adaptive functions for children with mucopolysaccharidosis I (Hurler syndrome) treated with hematopoietic stem cell transplantation.
• Management alternatives for vesicoureteral reflux.
• Of mice and men: design of a comparative anatomy information system.
• Ouabain decreases sarco(endo)plasmic reticulum calcium ATPase activity in rat hearts by a process involving protein oxidation.
• Oxidative modification of M-type K(+) channels as a mechanism of cytoprotective neuronal silencing.
• Paternity and hormone levels.
• Pediatric problems-from hydronephrosis to enuresis-discussed at AAP meeting: highlights from the american academy of pediatrics section on urology, october 17-19, 1998, san francisco, calif.
• Prenatal hydronephrosis-are voiding cystourethrograms necessary?
• Progress in islet transplantation in patients with type 1 diabetes mellitus.
• Stage-specific effects of cAMP signaling during distal lung epithelial development.
• Strategies for the Management of Exstrophy, Nonpalpable Testis, Hydronephrosis: Highlights From the Annual Meeting of the American Academy of Pediatrics Section on Urology October 9-12, 1999, Washington, DC.
• The risk of retractile testes becoming ascending testes.
• Twenty-six-year-old primigravid woman with a cystic mass.
• Update in pediatric urology: highlights from the annual meeting of the american academy of pediatrics section on urology november 28-29, 2000, chicago.
• Update on fetal surgery: highlights from the society for pediatric urology 49th annual meeting april 29, 2000, atlanta.
• Updates in Pediatric Urology: Highlights from the American Academy of Pediatrics Section on Urology Annual Meeting October 20-24, 2001, San Francisco, CA.
• Updates in Pediatric Urology: Highlights from the American Academy of Pediatrics Section on Urology Annual Meeting, October 18-21, 2002, Boston, MA.
• Updates in Pediatric Urology: Highlights of the American Academy of Pediatrics Section on Urology Annual Meeting, October 8-10, 2005, Washington, DC.
• Updates in Pediatric Urology: Highlights of the American Academy of Pediatrics Section on Urology Annual Meeting, October 8-11, 2004, San Francisco, CA.
• Updates in pediatric urology: highlights from the american academy of pediatrics section on urology annual meeting, october 31-november 3, 2003, new orleans, la.