Osteoprogenitor cells within skeletal muscle.

Osteoprogenitor cells within skeletal muscle. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Osteoprogenitor cells within skeletal muscle. Abstract Text:

P Bosch,D S Musgrave,J Y Lee,J Cummins,T Shuler,T C Ghivizzani,T Evans,T D Robbins, Huard,

The formation of ectopic bone within skeletal muscle is a widely observed phenomenon. However, the source of the osteoprogenitor cells responsible for ectopic bone formation remains unknown. This study was designed to test for osteogenic differentiation among cells isolated from skeletal muscle tissue. Different subpopulations of cells derived from an adult mouse skeletal muscle were tested for induction of alkaline phosphatase activity after exposure to bone morphogenetic protein-2 in vitro. A responsive subpopulation was identified, transduced with a retrovirus encoding for beta-galactosidase (Rv-lacZ) and an adenoviral construct encoding for one bone morphogenetic protein-2, and injected into the hindlimb of immune compromised (severe combined immunodeficient, or SCID) mice. The injected cells appeared to actively participate in the ectopic bone formation. The existence of lacZ-positive muscle-derived cells colocalized with osteocalcin-producing cells within lacunae of newly formed bone matrix suggests osteoblast and osteocyte differentiation. Although a specific cell was not isolated, these data support the contentions that osteoprogenitor cells reside within skeletal muscle and that muscle may represent a source other than bone marrow for the harvest of these cells.

Osteoprogenitor cells within skeletal muscle. Publishing Authors By Initials

P Bosch,DS Musgrave,JY Lee,J Cummins,T Shuler,TC Ghivizzani,T Evans,TD Robbins, Huard,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

PUBMED ID PMID:

MEDLINE DATE:

Osteoprogenitor cells within skeletal muscle. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of orthopaedic research : official publica

VOLUME: 18

Page Numbers: 933-44

Journal Abbreviation: J. Orthop. Res.

ISSN: 0736-0266

DAY: 19

MONTH: Nov

YEAR: 2000

Osteoprogenitor cells within skeletal muscle. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8404726

Osteoprogenitor cells within skeletal muscle. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: methods

Chemical & Substance for Abstract: Osteoprogenitor cells within skeletal muscle. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Osteoprogenitor cells within skeletal muscle.

AFFILIATION: Department of Orthopaedic Surgery, Musculoskeletal Research Center, University of Pittsburgh and Children's Hospital of Pittsburgh, Pennsylvania 15261, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: 1P60 AR44811-01

ACRONYM: AR

MEDLINETA: J Orthop Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Osteoprogenitor cells within skeletal muscle Related Publications

• A relationship between vascular endothelial growth factor, angiogenesis, and cardiac repair after muscle stem cell transplantation into ischemic hearts.
• A role for cell sex in stem cell-mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency.
• Adenovirus-mediated direct gene therapy with bone morphogenetic protein-2 produces bone.
• Clonal isolation of muscle-derived cells capable of enhancing muscle regeneration and bone healing.
• Decorin gene transfer promotes muscle cell differentiation and muscle regeneration.
• Dominant negative Bmp5 mutation reveals key role of BMPs in skeletal response to mechanical stimulation.
• Effect of bone morphogenetic protein-2-expressing muscle-derived cells on healing of critical-sized bone defects in mice.
• Enhancement of bone healing based on ex vivo gene therapy using human muscle-derived cells expressing bone morphogenetic protein 2.
• Ex vivo gene therapy to produce bone using different cell types.
• Growth factors improve muscle healing in vivo.
• Human skeletal muscle cells in ex vivo gene therapy to deliver bone morphogenetic protein-2.
• Interaction between macrophages, TGF-beta1, and the COX-2 pathway during the inflammatory phase of skeletal muscle healing after injury.
• Malignant transformation of multipotent muscle-derived cells by concurrent differentiation signals.
• Muscle-based gene therapy and tissue engineering.
• Muscle-derived stem cells for tissue engineering and regenerative therapy.
• Osteogenic potential of postnatal skeletal muscle-derived stem cells is influenced by donor sex.
• Osteoprogenitor cells within skeletal muscle.
• Regenerative Medicine for the Musculoskeletal System Based on Muscle-derived Stem Cells.
• Regenerative medicine in orthopaedic surgery.
• Report of case of Löffler's syndrome with a concomitant nonspecific maculo-papular rash.
• Sex differences in muscle-derived stem cells and skeletal muscle.
• Skeletal muscle fiber type conversion during the repair of mouse soleus: potential implications for muscle healing after injury.
• The effect of bone morphogenetic protein-2 expression on the early fate of skeletal muscle-derived cells.
• The efficiency of muscle-derived cell-mediated bone formation.
• [Cellular therapy can be affected by the sex of the stem cell used]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]